Product datasheet
Anti-CBR1 antibody ab4148
1 Abreviews 11 References 4 Images
Overview
Product name
Anti-CBR1 antibody
Description
Goat polyclonal to CBR1
Tested applications
ICC/IF, WB, IHC-P
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide: HGQFVSEKRVEQW, corresponding to C terminal amino acids 265-277 of
Human CBR1.
Run BLAST with
Run BLAST with
Positive control
Human Liver lysate.
General notes
Principal Names - CBR1; CBR; carbonyl reductase 1; carbonyl reductase (NADPH); carbonyl
reductase (NADPH) 1 Official Gene Symbol - CBR1 GenBank Accession Number –
NP_001748 LocusLink ID - 873 (human) Gene Ontology terms - carbonyl reductase (NADPH)
activity; metabolism; cytosol; oxidoreductase activity.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab4148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
ICC/IF
Abreviews
Notes
Use a concentration of 5 µg/ml.
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Application
Abreviews
WB
Notes
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 30 kDa.Can
be blocked with Human CBR1 peptide (ab22991).
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via
the microwave method before commencing with IHC staining protocol.
Target
Function
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide
variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various
xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and
daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert
prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher
affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Cellular localization
Cytoplasm.
Anti-CBR1 antibody images
Predicted band size : 30 kDa
ab4148 staining (0.2µg/ml) of Human Liver
lysate (RIPA buffer, 30µg total protein per
lane). Primary incubated for 1 hour. Detected
by western blot using chemiluminescence.
Western blot - CBR1 antibody (ab4148)
Ab4148 (3ug/ml) staining human CBR1 in
human kidney by immunohistochemistry using
paraffin embedded tissue.
Microwaved antigen retrieval with citrate
buffer pH 6, HRP-staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - CBR1 antibody
(ab4148)
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ICC/IF image of ab4148 stained HeLa cells.
The cells were 4% formaldehyde fixed (10
min) and then incubated in 1%BSA / 10%
normal donkey serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab4148, 5µg/ml) overnight
at +4°C. The secondary antibody (green) was
Alexa Fluor® 488 donkey anti-goat IgG (H+L)
Immunocytochemistry/ ImmunofluorescenceCBR1 antibody(ab4148)
used at a 1/1000 dilution for 1h. Alexa Fluor®
594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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Lanes 1 & 10 : Protein Marker
Lanes 2 - 9 : Anti-CBR1 antibody (ab4148)
at 1/1000 dilution (in PBS + (0.05%) Tween at
25°C )
Lane 1 : Protein Marker
Western blot - Anti-CBR1 antibody (ab4148)
Lane 2 : Whole cell lysate of human lung
cancer cell line A549 at 25 µg
Lane 3 : Whole cell lysate of human lung
cancer cell line A549 at 25 µg
Lane 4 : Whole cell lysate of human lung
cancer cell line A549 at 25 µg
Lane 5 : Whole cell lysate of human lung
cancer cell line A549 at 25 µg
Lane 6 : 1X SDS Loading Buffer
Lane 7 : recombinant CBR1 at 0.03 µg
Lane 8 : recombinant CBR1 at 0.07 µg
Lane 9 : recombinant CBR1 at 0.1 µg
Lane 10 : Protein Marker
Secondary
Lanes 2 - 9 : An HRP-conjugated anti-goat
IgG at 1/10000 dilution
developed using the ECL technique
Predicted band size : 30 kDa
Observed band size : 30 kDa
Exposure time : 1 minute
This image is courtesy of an anonymous
Abreview
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