Hypertension and the Metabolic Syndrome in Oman Intervention Clinical Trial and Mapping of Genes Project Code: Funds: Funding Source: Start Date: Duration: Team Members: (SR/MED/PHYS/04/01) 190,000 OMR His Majesty Trust Fund and NIH, USA 02 May 2004 4 years Researchers Prof. Mohammed Osman Hassan, P.I Prof. Riad Bayoumi, Co P.I The Hon. Dr. Sulayma Amur Albarwani The Hon. Dr. Ali Talib Al Hinai The Hon. Dr. Ali Jaffer Mohammad Mr. Saleh Said Al-Hadabi Dr. Jawad Ahmed Al Lawati Dr. Deepali Jaju Dr. Syed Rizvi Dr. Anthony G Comuzzie Research Assistants Graduate Students Mr Mohammed Nasser Al- Kindi Ms. Hameeda Sarhan Al Barwani Dr. George Khaukha Ms. Zahra Khalfan Al Busaidi Mr. Abdullah Hamed Al Harasi Ms. Johara Ali Salim Al Harasi Ms. Zaina Salam Al Hadhrami Mr. Hamad Saif Al Mundhari Ms. Saada Said Ali Al Riyami Mr. Fahad Mahmoud Al Zadjali Ms. Sawsan Salim Al Sinani Ms. Ghalia Mohammed Al Kasbi Ms. Fatma Jaffer Al Lawati Executive Summary Objectives The aim of this study was to find the prevalence of hypertension and the metabolic syndrome, identify known and other emerging risk factors and establish lifestyle protocols. Use the model of homogenous, consanguineous and large extended pedigrees to increase the statistical power of the heritability and linkage. Collect the maximum possible number of phenotypes (disease parameters) involved in the pathogenesis of hypertension and the metabolic syndrome using state-of- the art technology. Estimate heritability of all phenotypes to determine genetic and environmental contribution of each of the phenotypes in the pathogenesis of hypertension and the metabolic syndrome. Genotype all cohort subjects using modern human genome maps and Perform Linkage analysis of genotypes and phenotypes of each individual to detect quantitative trail loci ( QTLs) Perform fine genetic mapping for single nucleotide polymorphisms (SNPs) in QTLS with high LOD scores using modern microarray methods . Methods Six large, extended and highly consanguineous families were selected. The numbers of subjects in these 6 pedigrees were 310, 145, 226, 275 and 250, 293 totaling subjects. With the exception of pedigrees from Al Jabal Al Akhdar, All other 5 pedigrees sampled live within a perimeter of 20 km around Nizwa Anthropometric data, fasting and 2-hour glucose and insulin, fasting lipids, hormones profile, blood chemistry, liver enzymes were collected. Detailed haemodynamic and cardiac parameters and their indices were obtained during rest, cold pressor, mental (word conflict) stress tests and their reactivity using non-invasive beat-to-beat B.P measurements and impedance cardiography incorporated in the Task Force Monitor Unit (TFM, CNS systems). The following tests were performed after 10 minutes of rest, after 3 minutes of a Stroop word conflict and cold pressor tests. 24-hour Ambulatory blood pressure was recorded (Schiller BR 102). DNA samples for 1277 participants were initially genotyped using 10 cM genome-wide scan by the Mammalian Genotyping Services at Marshfield Clinic Research Foundation. The mean sexaveraged distance between adjacent markers was 8.6 + 6.5 cM with a predicted marker heterozygosity of 0.74 + 0.11. Linkage of the genotypic information and their statistics were conducted in the department of Genetics, Southwest Foundation for Biomedical Research (SFBR), San Antonio, Texas, USA. Results Prevalence of diseases and the population of the 5 Nizwa village the prevalence of hypertension was 31%, diabetes 5.5 %, high cholesterol 44% and the metabolic syndrome was 22%. In contrast the prevalence of the same parameters in the population of Al Jabal Al Akhder was 4%,2%,,35%, and 2%, respectively One of the most important findings of this study were the prevalence of prediabetes or impaired glucose tolerance (IGT) was 12% The prevalence of borderline in young offspring of one or two hypertensive parents is 31% The prevalence of the metabolic syndrome in females was twice as much than in males. Heritability of high cholesterol, body fat and body mass index was mainly due to genetic factors while that of blood sugar and waist circumference were mainly due to environmental factors. Gene Loci (QTLs) detected were 14 signals with LOD score above 3.00 and 58 signals with LOD scores 2.00 -3.00 QTLs with LOD scores above 3 for body mass index, weight, leptin and body fat and insulin were found in the same areas in chromosomes 1 and 3. QTLs with LOD scores above 3 were found for heart rate, cardiac index stroke index and cardiac contractility were detected during mental stress in different chromosomes. Conclusions The important findings in this study were that the prevalence of the metabolic syndrome was twice as high in females than males. In the whole study population the prevalence of pre-diabetes and borderline hypertension was significantly higher than that of diabetes and hypertension, respectively. While heritability of high cholesterol , body mass index and body fat was mainly due to genetic factors, that of blood sugar, triglycerides and waist circumference were mainly due to environmental factors The Significant QTLs for cholesterol and body mass index were replicated in other studies. QTLs for blood pressure for its intermediate phenotypes were not reported before and were obtained during laboratory stress tests. Laboratory mental stress, which revealed the only significant blood pressure QTLS, may represent a replay of the environmental conditions that contribute to the development of essential hypertension may help identify the genes of this complex condition. In addition, the different loci detected during rest, mental, physical stress and during reactivity in the whole genome further confirm the complexity of BP control as well as the oligogenic epistatic nature of the genetic components of blood pressure determination. Al Jabal al Akhdar Initiative – Conservation and Sustainable Development in a fragile mountain ecosystem in northern Oman Project Code: Funds: Funding Source: Start Date: Duration: (SR/AVP/CESR/04/01) 65,850 OMR His Majesty’s Trust Fund 1 September 2004 3 years and 4 months Team Members Researchers Prof. Reginald Victor (PI) Dr. Michael Robinson(Co PI) Dr. Osman Mahgoub Gaafar Dr. Annette Patzelt Dr. Atsu. Dorvlo Dr. Jens Eriksen Dr. A. Pillay Dr. Mushtaq Ahmed Dr. Malik Mohamed Al-Wardy Dr. David Ampratwum Dr. Dipak Chaudhuri Dr. Ramanathan Dr. Geetha Subramanian Dr. Andy Kwarteng Research Assistants Mr. Mansour Hamed Al-Haddabi Mr. Juma Khalfan Al-Handhaly Mr. Abdulla Mohammed Al-Shuraqi Mr. Hamed Hareb Al-Riyami Mr. Ahmed Mohammed Al-Wahaibi Mr. Abdurahman Suleiman Al-Hinai Mr. Tamim Hamed Al-Haddabi Ms. Leila Said Al-Harthi Executive Summary Objectives Al Jabal Akhdar Initiative was a strategic research project that addressed a major issue of wise natural resource utilization and sustainable development under the guiding principle of the National Biodiversity Strategy and Action Plan (NBSAP) Oman. This multidisciplinary project built together a foundation of core environmental and socioeconomic issues to promote a synthesis of conservation and sustainable development in this area, at present under severe stress caused by urban and tourism development. Methods Since this project was made up of nine subprojects: climate, biodiversity inventory, flora and vegetation, water resources, soil resources, livestock diet and range conditions, goat husbandry systems, ecotourism development and socioeconomic impact assessment of tourism development, a wide variety of scientific methodologies depending on the issues investigated were used. These included collection and analysis of data from primary and secondary sources for climate and biodiversity inventory, ecological methods for assessment of vegetation, analytical methods for estimating physical and chemical parameters in soil and water, evaluation of livestock diets, standard methods used in animal husbandry, survey methodology for socioeconomic studies and mathematical modeling. Results Climatic changes on Saiq Plateau were evaluated using meteorological data for the period 1987 to 2004. The data were analyzed for trends and, changes in pattern. Models were developed for some parameters. There is a temperature increase and the indirect precipitation is reducing. If these conditions persist, the ecosystem on the Plateau would be affected adversely. The biodiversity inventory recorded 437 species excluding the flora and birds. A total of 77 bird species were recorded including nine species new to areas bringing the total number of recorded species to 129. About one-fourth of the total flora of the country is found in Jabal Akhdar range above 1500m in the Olive-Juniper woodlands (Olea europaea subsp. cuspidata-Juniperus excelsa subsp. polycarpos). The mountains exhibit high species diversity and represent a key biodiversity region in the Arabian Peninsula. The water resources of the area were investigated specifically addressing the water quality and water use efficiency. Eutrophication is a common phenomenon and the surface water is not fit for human consumption. The groundwater resources are over exploited. Soil samples were analyzed for soil texture, pH, electrical conductivity (EC), and water soluble and aqua regia digestible elements. Results recommend appropriate land use for agriculture and sustainable exploitation of the natural vegetation for the benefit of local communities. The Saiq Plateau is probably the most intensely used animal grazing area in the western Hajar Mountains of Oman. This study has quantified the livestock diets and the degree of plant resource sharing. The grazing intensity in these mountains is beyond sustainable levels. The evaluation of goat husbandry systems recommends pen feeding as opposed to free ranging to increase animal productivity. This study also focused on optimizing the benefits of ecotourism in Jabal Akhdar and the results provide general guidelines to the decision makers for enhancing the ecotourism activities in the region. The socio-economic impact assessment (SEIA) due to tourism development identified socio-economic costs and benefits for the region and quantitatively estimated the impacts in some cases. A comprehensive Socioeconomic Environment Management Plan (SEEMP) that would minimize the negative impacts and maximize the positive impacts is developed. Conclusions Al Jabal al Akhdar is a fragile arid mountain ecosystem experiencing the effect of increasing temperature and decreasing precipitation. It is severely stressed by the rapid urban development including tourism. All natural resources including biodiversity, vegetation, water, soil, and agricultural and animal resources are impacted. Ecotourism in the region need to be optimized and socioeconomic impacts of tourism need to be mitigated. Strategies for the conservation of this ecosystem need to be developed and unsustainable activities affecting ecosystem services must be checked. Otherwise, this biodiversity hotspot in the Arabian region will be lost irretrievably. Development of Information Technology Training and Research Infrastructure Project Code: Funds: Funding Source: Start Date: Duration: Team Members: (SR/ENG/ECED/04/01) 184,100 OMR His Majesty’s Trust Fund 01 December 2003 5 years Researchers Dr. Ahmed Mohamed Al-Naamany Dr. Hadj Bourdoucen Dr. Joseph A. Jervase Research Assistants Graduate Students Mr. Mohamed M. Elzain Ms. Aamna Abdullah Al-Sinani Mr. Mohamed Teirab Mr. Wadeh Hafidh Al-Menthari Mr. Ali Amer Al Shidhani Mr. Ahmed Hammad Al-Kalbani Mr. Salim Ali Al-Nasseri Mr. Adel Said Al-Balushi Executive Summary Objectives The main objective is to develop an infrastructure for dissemination of IT know-how in Oman by training the trainers, for the advancement of the nation’s overall manpower development in the public and private sector. The secondary objective is establishment of an IT research base at SQU to eventually serve the specific needs of Oman in all related aspects of IT. Methods A literature review for assessment of state-of-the-art networking technologies, including integrated networking and converged networking was initially undertaken. This was followed by a study of the existing national IT Infrastructure. The sectors of the economy where IT serves as a productivity multiplier were identified and means to increase IT training for these sectors was proposed. Furthermore, the segment of the population, which is in a position to transfer IT knowhow to others was identified. A training plan based on the Sultanate's needs was subsequently laid out. This involved setting of IT courses and offering special training to the identified sector of the population. Results Individuals that will have an impact on the nation overall manpower development in the public and private sector are the teachers. Based on this, over 250 primary and secondary teachers from the Ministry of Education and Ministry of Higher Education were trained. The courses undertaken were two (1) Information Technology Essentials I: PC Hardware & Software (2) Information Technology Essentials I: Network Operating Systems. Raising the level of the teachers in the IT field, will have impact on quality and level of education. An IT facility was developed by purchasing, installing and testing hardware equipment and simulation packages for the IT facility. This facility currently provides training for SQU students as well as for public and private sectors in the field of IT research and testing of new systems. Conclusions Over 250 school teachers from the public schools covering the various regions of Oman have been successfully trained on modern Information Technology techniques. A high quality IT facility has been developed and established at SQU for training as well as research. Surfactants for Enhanced Oil Recovery in Yibal Reservoir conditions: Product selection and Evaluation Project Code: Funds: Funding Source: Start Date: Duration: Team Members: (SR/SCI/CHEM/04/01) 35,950 OMR His Majesty’s Trust Fund 02 December 2004 4 years Researchers Dr. Mohamed Hassen Aoudia (P.I) Dr. Rashid Salim Al-Maamari Research Assistants Mr. Moein Nabipour Ms. Badria Yahia Al-Busaidi Mr. Aadel Awadh Al Dukhli Mr. Hamdan Saif Al-Amri Mr. Nayar Al-Afzal Mr. Ike Siruno Mr. Mohamed Nasser Al-Shibli Mr. Ali Rashid Al-Hinai Graduate Students Ms. Amal Hilal Al-Sabahi Ms. Zuleikha Said Al-Harthi Mr. Said Mohamed Al-Faraji Ms. Zakia Hilal Al Maawali Executive Summary Objectives Many Omani oil fields reached the second stage of production (water injection) and evidence is that enhanced oil recovery (EOR) techniques may be needed to increase oil production in the future. Therefore, the main objective of this project is to formulate and optimize surfactant systems suitable for Yibal field conditions. A rigorous approach in selecting suitable modified nonionic surfactants was undertaken, based on extensive empirical correlations between surfactant structure on one hand, and reservoir conditions on the other. Clearly, this crucial issue to extend Omani oilfield life is of paramount significance to the economy of Oman and therefore may contribute to the energy supply in the future. Methods The design of surfactant flood system depends on the following reservoir characteristics: brine composition, crude oil nature, reservoir temperature, reservoir rock, porosity, and permeability. Because of their excellent tolerance to high salinity, modified nonionic surfactants were considered in this investigation. Surfactant selection was based on the following screening criteria in reservoir conditions (salinity and temperature): i) Compatibility between brine and surfactant, ii) –3 -2 Ultralow interfacial tension at the brine-10 dynes/cm) to capillary forces between brine and entrapped droplet of oil, iii) Long term stability of the surfactant slug in the reservoir, iv) low surfactant adsorption on reservoir rock, and v) Additional residual oil recovery by surfactants. Results A series of modified nonionic and amphoteric surfactants showed excellent compatibility with reservoir conditions (salinity and temperature) and can therefore be safely injected in Yibal reservoir.(IFT) measurements were carried out at different surfactants concentrations in reservoir tr eq) in a low and wide range of surfactant concentrations. These two properties are highly desirable in enhanced oil recovery field applications. The selected surfactants can therefore be potential candidates for actual surfactant flood in Yibal oil field. IFT between crude oil and reservoir brine for a freshly prepared surfactant system as well as for the aged surfactant solution (five months) were measured.. Both systems display the same IFT behavior, suggesting that no degradation of the surfactant is occurring during the duration of the laboratory test (5 months). Adsorption isotherms were measured for AES-802, AES-805, and AES-808 surfactants in reservoir conditions (salinity and temperature). The adsorption was found to be 20 mg/g, 5 mg/g, and 5 mg/g, respectively. To make the surfactant flood process economically viable, surfactant adsorption must be reduced to around 0.1-0.5 mg/g. Current research is on progress in order to achieve this condition. Surfactant injections after waterflooding were conducted (tertiary surfactant injection). In all cases additional increase in oil recoveries between 1% and 7% of OOIP were achieved. Highest additional oil recoveries were generated with 7-58 (7%), AES-506 (6%), AES-208 (7%), and 6-105 (4%) surfactants, whereas relatively lower additional oil recoveries were obtained with AES-218 (1%), and AES-128 (1%) surfactants. Conclusions Modified nonionic surfactants were selected and evaluated for their potential use in enhanced oil recovery by surfactant flooding in Yibal oil field. A series of suitable surfactants showed a -2 complete compatibility with reservoir conditions, an ability to reduce the IFT to less than 10 -1 mNm , to be stable during reservoir flooding, and to generate residual oil recovery. However, the adsorption of surfactant onto the reservoir rock was found to be relatively high. Our efforts will focus on hoe to reduce this adsorption. Improving Production of Vegetable Crops by Development of Stress Tolerant Germplasm and Farming Systems Research Project Code: Funds: Funding Source: Start Date: Duration: Team Members: (SR/AGR/PLNT/04/01) 74,800 OMR His Majesty’s Trust Fund 02 May 2004 5 years Researchers Research Assistant Dr. Fahad Aljulanda Al-Said (P.I.) Dr. Iqrar Ahmed Khan (P.I) Dr. Mike Deadman Dr. Abdullah Mohammed Al-Sa'di Dr. Amnat Ali Dr. Slim Zekri Dr. Sanmugam Prathapar Dr. Nerru B. Bhat Mr. Khamis Ahmed Al-Habsi Mr. Yusaf Mohammed Al-Maqbali Mr. Aziz Hmaed Al-Rawhai Mr. Khalid Abdullah Al-Kaf Mr. Murie Salim Al-Barhi Mr. Khalid Abdulaziz Al Farsi Mr. Ali Said Al- Nabhani Mr. Issa Saleh Al-Forqani Mr. Ali Naifer Mr. Shafat Ali Sadiq Graduate Students Mr. Khalid Abdulaziz Al-Farsi Ms. Tina George Mr. Mahmood Al-Nabhani Ms. Akino Ohashi Mr. Abdullah Mohammed Al-Sa'di Executive Summary Objectives This project aims to improve vegetable production in Oman and break the stagnation in production by expanding vegetable production, extending seasons of production and generating higher yields through international collaboration for germplasm acquisition and testing for local adaptations, quality characteristics and seed multiplication with emphasis on tomato and melon, and transfer this technology to farmers through Ministry of Agriculture (MA). It also looks at management of late season vine decline of melon and other cucurbits, and to determine the specific nature of diseases limiting production. The project encompasses establishment of a database on farming systems of Oman including costs of production, and identifies system-based handicaps to temporal expansion of production including water-use efficiency by different vegetables compared with hey grass. Methods In the first phase of the project, seed/germplasm was acquired from the Asian Vegetable Research and Development Center, Taiwan and the University of California, Riverside through material transfer agreements (MTAs). Experimental work was performed in order to: 1) study yield and fruit characteristics of tomato and pepper lines, 2) investigate disease aspects of melon and other cucurbits through field testing of varieties and disease control measures (crop rotation, utilization of crop residues, solarization and chemical control) for late season vine decline (LSVD) of melon crops and 3) determine the specific nature of diseases limiting production. The research was undertaken at 3 locations: AES/SQU, Rumais/MA and Barka/commercial farm. Water-use efficiency by different vegetables during different growing seasons was conducted with special reference to year-round productivity and in order to compare field and greenhouse/shadehouse produced vegetables. The study utilized water measuring devices (flow meters) in 24 field plots where crops including potato, tomato, pepper, melon, cucumber, corn, beans and cabbage were grown. Water use and yields were recorded for each crop in all growing seasons. Finally, research was undertaken to investigate the farming systems of Oman including costs of production, in order to compile a marketing database from a survey of 100 farms. Results Different germplasm lines of tomato showed varying levels of adaptability to cultivation conditions in Oman. This indicates the potential for some varieties, in future, to be propagated in Oman as part of a seed production industry. The farming system survey, including costs of production and marketing analysis confirmed the pre-eminent status of vegetables, with tomatoes the most important component, followed by melons. The analysis showed that the economic return from vegetable production is higher than that from fruit crops, but lower than from fodder crops. However, in terms of productivity relative to water consumption and tonnage of food produced per unit land area and time, vegetables appear to perform better than other crop types. Field examination of LSVD of sweet melon and other soil-borne diseases of cucurbits indicated that the causal factors in the development of LSVD are complex, including physiological stresses imposed on the crop. Research on soil-borne diseases of cucurbits revealed the presence in Oman of three new fungal pathogens. The incomplete management of LSVD led directly to a new SQU/MA collaborative project on the use of grafting for the management of this disease as well as of greenhouse cucumber diseases. In addition, the research showed the importance of strengthened quarantine regulations in the management of crop diseases, indicating the potential for imported potting mixes to bring into Oman new fungal pathogens. Conclusions Continuous introduction of new varieties through collaboration with international institutions like the Asian Vegetable Research and Development Center, Taiwan and technology transfer in vegetable production in Oman is necessary to improve vegetable production. Furthermore, introduction of new technology such as grafting to farmers and strengthened quarantine regulations in the management of crop diseases is an effective way to improve vegetable production and to reduce the impact of wilt diseases in cucurbits and to prevent entry of new disease to Oman. Finally, periodical updating of the developed database of Agricultural Farming systems in Oman is critical to identify handicaps and future changes in the vegetable production sector specifically and the crop production sector in general. Coastal Erosion in Al Batinah Project Code: Funds: Funding Source: Start Date: Duration: Team Members: (SR/ART/GEOG/04/01) 69,050 OMR His Majesty’s Trust Fund 02 May 2004 4 years Researchers Dr. Salim Mohamed Al-Hatrushi (PI) Dr. Andy Yaw Kwarteng (Co-PI) Dr. Ahmed Sana Prof. Anton MacLaclan Dr. Khalid Hamed Dr. Ali Said Al-Buloushi Consultants Research Assistants Dr. G. T. Vijaya Kumar Dr. Mohammed Aslam Abdul Khader Mr. Abdulhameed Al-Riyami Mr. Jamil Said Al-Yaqoubi Dr. G. T. Vijaya Kumar Dr. Mohammed Aslam Abdul Khader Mr. Abdulhameed Mohamed Al-Riyami Mr. Jamil Said Al-Yaqoubi Graduate Students Mr. Ahmed Hassan Al-Naabi Mr. Ahmed Saif Al-Dhuhli Mr. Saleh Ali Al-Hasani Dr. Werner Kurt Illenberger Executive Summary Objectives Al Batinah coastal area has been experiencing rapid development over the past three decades including the construction of major engineering infrastructures such as harbors, corniches, roads, etc. Coastal erosion is observed in some areas and the construction of engineering structures are considered to be a contributing factor. This project aimed to assess the status of coastal erosion in Al Batinah using a series of integrated studies and to suggest solutions. Methods A multidisciplinary approach was adopted in order to address the issue of beach erosion along Al Batinah coast as holistically as possible. Accordingly, the study included the following sections: (1) Literature review of existing knowledge, (2) Survey of the needs, problems and perceptions of local communities concerning sandy beaches, (3) Use of aerial photos and satellite imagery to consider large scale changes to this whole section of coast, (4) Regular surveys of beach profiles at selected locations to initiate a monitoring system and to obtain measurements on a finer scale (temporal and spatial) of changes, (5) Analysis of grain sizes and mineralogy of the sediments in order to understand the origins of this material and to enable the use these properties as tracers in future, (6) Estimates of the extent to which terrestrial sediment supply to these beaches may have been reduced through sediment trapping in recharge dams on wadis draining the western Hajar Mountains, and (7) Making provisional measurements of wave driven currents and nearshore circulation and modeling the role of these processes in transporting sand. Results The questionnaire survey showed overwhelming dependence on marine resources and beaches, more than 50% of respondents being directly involved in fishing for employment and 97% indicating that beaches were very important for their subsistence and lifestyle. Aerial photos and satellite images revealed that on the large scale and over several decades the soft shoreline of Al Batinah coast was stable. Some local accretion and erosion had occurred on up- and down drift-sides of major coastal engineering structures (especially Widam Sahel) and at the mouth of Wadi Hawasnah, but otherwise stability was the norm. Most profiles showed some variability but overall there was stability over two years of monitoring. The cases of accretion or erosion were all closely associated with harbor and other coastal engineering structures and no cases were extreme. Grain size distribution and mineralogy of sediment on Al Batinah coast showed a wide range of sizes, types and origins, but the main source was terrestrial, from the western Hajar Mountains. Total wadi sediment input to Al Batinah coast was estimated at 1,560,000 m³ per year historically, coming almost entirely from the western Hajar Mountains. Recharge dams now trap 48% of this, significantly reducing sediment input to the shore. The in situ nearshore measurements by ADCP recorded wave heights in the range 0.2-1.4 m, wave periods generally 4-5 seconds, and current speeds up to 0.5 m per second. Conclusions The overall conclusions from this integrated series of studies are that: (1) Al Batinah sandy shoreline is generally stable, (2) Local interruption of longshore transport by coastal engineering structures has caused limited erosion and accretion on short temporal and spatial scales, (3) Reduction of wadi sediment supply does not yet seem to have had major effect, with the exception of Wadi Hawasnah, (4) Further work on modeling will allow more accurate budgets to be compiled and it is desirable that monitoring should continue, (5) Local people are dependent on good beaches and are keenly aware of local erosion problems, (6) In most cases the local population can live with these problems but in some areas nourishment could be tried. Sediment cleaned from dams could also be brought to the coast, and (7) It is most important that a national body be set up to link all interested and affected parties, disseminate information and continue monitoring. Environmental Assessment of Selected Waste-Dumping Sites in Oman and Risks on Populations Living in their Vicinity (Case Study: Barka and Bowsher) Project Code: Funds: Funding Source: Start Date: Duration: Team Members: (SR/ENG/CAED/04/01) 94,350 OMR His Majesty’s Trust Fund 02 May 2004 4 years Researchers Dr. Ahmed Mohamed Al-Futaisi (P.I.) Dr. Ahmad Jamrah Dr. Saif Nasser Al-Bahry Dr. Abdulgadir Elshafie Dr. Salim Said Al-Wahibi Dr. Majed Shahoo Al-Zedjali Eng. Said Darwish Al-Alawi Eng. Haitham Khalfan Al-Akhzami Research Assistants Dr. Natarajan Rajmohan Mr. Said Mohammed Al-Touqi Mr. Saif Muhana AL-Yaroubi Graduate Students Mr. Said Mohamed Al-Touqi Executive Summary Objectives The main objective of this research is to develop a quantitative environmental risk assessment strategy using the source-pathway-receptor framework for some selected waste dumping sites in Oman and to identify possible contamination of the unsaturated zone and aquifers that exist beneath waste dumping sites. Two landfill have been selected as case studies: Barka Dump and Bowsher. Methods Detailed data collection was carried out from various public and private sources. Related data were transferred to spatial maps using GIS. About five dumping sites were visited in order to select two sites for more comprehensive investigation. It was decided that the project will concentrate on Barka and Bousher dump-sites. During the study period, regular visits to the landfills were carried out for monitoring and quantification of the wastes in the dump sites. Many waste samples were collected and analyzed at SQU labs for diverse microbiological, chemical, and environmental parameters. Observing great potential of groundwater contamination from Bark dumping site, groundwater quality of the entire city was tested for any possible environmental pollution from the dumping sites or other sources. The relation between rainfall and groundwater potential was evaluated from the groundwater level and rainfall data related to Barka collected from 1984 to 2003. More than hundred samples were collected, analyzed and presented on GIS spatial maps to show the contamination plumes observed in Barka aquifer. Geochemical Analysis was performed on Barka groundwater for understanding the chemical processes involved in Barka aquifer. The study implemented geophysical method using Time-Domain Electro Magnetic (TDEM) for identifying possible migration of contaminants from the surface to the unsaturated zone below the site and hence to groundwater. A total of five boreholes were drilled up to a depth of 80 meter in order to obtain soil and groundwater samples from beneath Barka dumping site to investigate the possibility of contamination of the groundwater in Barka aquifer. Results Waste and soil samples collected from Bowsher and Barka dumping sites suggested that the soils are heavily polluted with organics especially Total Petroleum Hydrocarbons (TPHs), a range of toxic metals and nutrients. The continuous discharge of effluents resulted in the creation of many stagnant ponds and estuaries flowing towards the lower lands providing luxurious and favorable breeding sites for mosquitoes, flies, worms, and other microorganisms. Results of Barka water level analysis illustrated that groundwater wells in Barka exhibit distinctive interesting trend in the long-term water level fluctuations. The long-term regional variation in water level revealed that water level continuously dropped down in the coastal and centre part of Barka with an annual decline in water level of 0.3 to 0.4 m/year. In Barka, groundwater chemistry showed large variations in mean (or median) values and also expressed high standard deviations in TDS, Cl, Na, Mg and SO4, which suggest the influences of distinguished contamination sources and/or process on water chemistry. Trace metals expressed distinguished distribution pattern from upstream to downstream. Elevated concentrations of metals such as Zn, Fe, Mn, Cu, Cd, and Pb were observed near the dumping site and decrease along with groundwater flow. Bacterial population was generally high near dumping site and coastal strip. Overall, 52% of samples exceeded anyone of the inorganic parameters based on Oman standards while 98% of samples are unfit for domestic usage based on EPA standards. Total coliform and E. coli were observed in 90% and 69% of samples, respectively, which are not appropriated for regular uses. Plotted DRASTIC maps indicated that the northern and central parts of Barka are more vulnerable to pollution than southern part. Regional distribution pattern of nitrate, chloride, Total coliform and Fecal coliform were well correlated with DRASTIC vulnerability maps. Geochemical study suggested that the groundwater chemistry in Barka is principally influenced by saline sources, cation exchange, anthropogenic pollutants and mineral dissolution/precipitation. Results of TDEM study suggested that lower resistivity zone (<80 ohm m) encountered near the subsurface indicates plume migration caused by liquid waste disposal activities. Combination of TDEM survey results with lithology of peizometers indicated that higher resistivity is correlated with compacted/cemented gravels and cobbles, especially medium dense to very dense gravels and cobbles. It was observed that the some of the drilled wells at the site and close to the lagoons, contains elevated concentration of TDS, Na, Ca, Mg, alkalinity, chloride and total hardness. This observation suggested that groundwater below the dumping site is strongly affected by leachate originated from liquid lagoons located inside the landfill. Conclusions Characteristics of waste materials collected from Barka and Bousher dumping sites imply that codisposal of untreated solid, sludge and liquid wastes in one site has resulted in a severe trade-off in the eminence of environment. The solid waste samples collected from the dumping sites of both landfills confirmed the presence of a wide range of organic and inorganic contaminants. The soils are heavily polluted with organics especially TPH, a range of toxic metals and nutrients. Regional groundwater sampling and analysis in Barka confirmed that groundwater level is continuous declination and that groundwater chemistry is influenced by distinguished contamination sources and process. Trace metal contents firmly reveal the effect of dumping site on groundwater chemistry and most of the metals seem to be derived from liquid waste water disposal. GIS spatial maps of groundwater quality parameters indicate that entire groundwater aquifer in Barka city is bacteriologically contaminated and not fit for direct drinking or usage. Groundwater quality in Barka was evaluated using Oman standards for drinking water (OSTD) and USEPA standards and concludes that 98 % of samples are unfit for drinking based on salinity and bacteria. Impact of anthropogenic activities on groundwater composition in Barka is apparently recognized from nitrate concentration, originated from nitrification and/or organic matter decomposition, and increases solute load indirectly in groundwater. Groundwater samples collected from drilled pizometers at Barka landfill contains elevated concentration of TDS, Na, Ca, Mg, alkalinity, chloride and total hardness suggesting that groundwater below the dumping site is strongly affected by leachate originated from liquid lagoons located inside the landfill. Microbiological parameters show that groundwater beneath Barka dumping site is largely influenced by bacterial contamination especially Total coliform and E.coli.