Product datasheet
Urease Activity Assay Kit (Colorimetric) ab204697
3 Images
Overview
Product name
Urease Activity Assay Kit (Colorimetric)
Detection method
Colorimetric
Sample type
Urine, Adherent cells, Suspension cells, Purified protein
Assay type
Enzyme activity
Sensitivity
< 0.001 mU/well
Product overview
Urease Activity Assay Kit (Colorimetric) (ab204697) provides a quick and easy way to
determine urease activity. The kit uses a modified Berthelot method to detect ammonia, which
can be measured at 670 nm. The kit is suitable for measuring urease activity of biological and
soil samples as well as of purified enzyme. The limit of detection is below 0.001 mU.
Notes
Urease (EC 3.5.1.5) catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease
is found in certain bacteria, yeast, fungi, and plants. In addition, it is found environmentally in soil
samples and fecal matter. Due to ammonia production, urease can play a role in the
pathogenesis of certain medical conditions including urinary stones, peptic ulcers, and hepatic
encephalopathy. As such, urease can be used as a diagnostic for gastrointestinal and urinary
tract pathogens.
Tested applications
Functional Studies
Platform
Microplate
Properties
Storage instructions
Store at -20°C. Please refer to protocols.
Components
Identifier
100 tests
Ammonia Reagent 1
Amber
1 x 8ml
Ammonia Reagent 2
Clear
1 x 4ml
Ammonium Chloride Standard (100 mM)
1 x 100µl
Urea (50x)
1 x 1.5ml
Urease
1 vial
Urease Assay Buffer
1 x 15ml
Relevance
The enzyme urease catalyzes the hydrolysis of urea to create carbon dioxide and ammonia. The
protein consists of a single kind of polypeptide chain containing 840 amino acid residues. The
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subunit relative molecular mass calculated from the sequence is 90,770, indicating that urease
is composed of six subunits.
Cellular localization
Cytoplasmic
Applications
Our Abpromise guarantee covers the use of ab204697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Functional Studies
Use at an assay dependent concentration.
Urease Activity Assay Kit (Colorimetric) images
Typical ammonia Standard calibration curve
using Urease Activity Assay Kit (Colorimetric)
(ab204697).
Urease Activity Assay Kit (Colorimetric)
(ab204697)
Measurement of Urease activity in
Staphylococcus aureus and Saccharomyces
cerevisiae (yeast) cell extracts.
Urease Activity Assay Kit (Colorimetric)
(ab204697)
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Measurement of Urease activity in soil.
Urease Activity Assay Kit (Colorimetric)
(ab204697)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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