PeakFinder Quick Start Run the PeakFinder setup.exe program to install PeakFinder. After initial installation (or after deleting the file C:\Documents and Settings\<UserID>\Local Settings\Application Data\StowersInstitute\PeakFinder\PeakFinder.INI file), click on the PeakFinder desktop icon to start the program. (Or, select Start | Programs | Peakfinder to start the program. (Normally the installed program is in the C:\PeakFinder folder). This message will immediately appear: Click OK. Select button "1. Genome Index File". Select the file C:\PeakFinder\Yeast\YeastIndex.dat, and press the Open button. Select button "3. Coordinates" and select the file C:\PeakFinder\Coordinates.xls, and Open. Select button "4. Ratios" and select the file C:\PeakFinder\Ratios.xls, and Open. Select Chromosome III from the drop-down list. PeakFinderQuickStart.doc 2.25.2004 1 PeakFinder Quick Start Select the Multi-Row Chart tabsheet: Uncheck Show Raw Ratio Data: PeakFinderQuickStart.doc 2.25.2004 2 PeakFinder Quick Start Select the Single-Row Chart tabsheet. With Bitmap selected, you can immediately paste this chart into Word (or other programs): PeakFinderQuickStart.doc 2.25.2004 3 PeakFinder Quick Start Likewise, with Enhanced Metafile selected, the same chart can be pasted as a metafile into Word (or other programs): Ratio Chromosome III 9 2 4 13 16 14 5 21 12 3 1 Content(30 kb w indow ) GC 11 8 1 0 10 15 6 7 17 22 40 18 19 23 20 27 24 25 26 2 28 -1 35 PeakFinderQuickStart.doc 2.25.2004 4 PeakFinder Quick Start With Postscript selected, you cannot view the graphic in PeakFinder. Press the Save button and save the postscript (.ps) file to disk. PeakFinderQuickStart.doc 2.25.2004 5 PeakFinder Quick Start Open the postscript file using GSView, or other programs such as Adobe Illustrator: PeakFinderQuickStart.doc 2.25.2004 6 PeakFinder Quick Start Using Illustrator: PeakFinderQuickStart.doc 2.25.2004 7