Product datasheet
Anti-CDKAL1 antibody [EPR11621-26] ab169531
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Overview
Product name
Anti-CDKAL1 antibody [EPR11621-26]
Description
Rabbit monoclonal [EPR11621-26] to CDKAL1
Tested applications
WB, ICC/IF, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant fragment corresponding to residues in Human CDKAL1 (UniProt Q5VV42).
Positive control
HepG2, HeLa, Raji and MDA-MB-435 cell lysates; HeLa cells; Immunoprecipitation pellet from
HepG2 cell lysate.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class
antibodies. As a result of this work we are pleased to now offer this antibody in purified
format. We are in the process of updating our datasheets. The purified format is
designated "PUR" on our product labels. If you have any questions regarding this
update, please contact our Scientific Support team.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR11621-26
Isotype
IgG
Applications
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Our Abpromise guarantee covers the use of ab169531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IHC-P.
Target
Function
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the
formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs
that read codons beginning with adenine.
Tissue specificity
Expressed in pancreatic islets.
Involvement in disease
Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulindependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by
a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body
habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin
resistance, hypertension and hypertriglyceridemia. The disease results in long-term
complications that affect the eyes, kidneys, nerves, and blood vessels.
Sequence similarities
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
Contains 1 MTTase N-terminal domain.
Contains 1 TRAM domain.
Cellular localization
Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for
insertion into the endoplasmic reticulum.
Anti-CDKAL1 antibody [EPR11621-26] images
All lanes : Anti-CDKAL1 antibody
[EPR11621-26] (ab169531) at 1/1000
dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : MDA-MB-435 cell lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-CDKAL1 antibody [EPR11621-
Secondary
26] (ab169531)
HRP labeled goat anti-rabbit at 1/2000
dilution
Predicted band size : 65 kDa
2
Immunofluorescent analysis of HeLa cells
labeling CDKAL1 with ab169531 at 1/50
dilution. DAPI nuclear staining (blue).
Immunocytochemistry/ Immunofluorescence Anti-CDKAL1 antibody [EPR11621-26] (ab169531)
Anti-CDKAL1 antibody [EPR11621-26]
(ab169531) at 1/1000 dilution +
immunoprecipitation pellet from HepG2 cell
lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially
detecting the non-reduced form of rabbit IgG
Predicted band size : 65 kDa
Western blot - Anti-CDKAL1 antibody [EPR1162126] (ab169531)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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