Product datasheet
Anti-SIPA1 antibody ab10162
1 Abreviews 1 Image
Overview
Product name
Anti-SIPA1 antibody
Description
Goat polyclonal to SIPA1
Tested applications
WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide: ASKQLGSPTADLA, corresponding to C terminal amino acids 1030-1042 of
Human SIPA1.
Run BLAST with
Positive control
Run BLAST with
Human lymph node and Jurkat.
General notes
SIPA1 is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity
for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This
protein may also hamper mitogen-induced cell cycle progression when abnormally or
prematurely expressed. It is localized to the perinuclear region.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Primary antibody notes
SIPA1 is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity
for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This
protein may also hamper mitogen-induced cell cycle progression when abnormally or
prematurely expressed. It is localized to the perinuclear region.
Clonality
Polyclonal
Isotype
IgG
Applications
1
Our Abpromise guarantee covers the use of ab10162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
Use at an assay dependent dilution. Detects a band of approximately 130 kDa
(predicted molecular weight: 130 kDa).Can be blocked with Human SIPA1
peptide (ab23193).
Target
Function
GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro,
converting it to the putatively inactive GDP-bound state.
Tissue specificity
Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such
as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in
the spinal cord.
Sequence similarities
Contains 1 PDZ (DHR) domain.
Contains 1 Rap-GAP domain.
Cellular localization
Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the
perinuclear membraneous region.
Anti-SIPA1 antibody images
Predicted band size : 130 kDa
This image was submitted as part of a review
by Kathy Till
Western blot - SIPA1 antibody (ab10162)
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