Product datasheet Anti-HORMAD1 antibody [EPR10273] - C-terminal ab178432 1 Image Overview Product name Anti-HORMAD1 antibody [EPR10273] - C-terminal Description Rabbit monoclonal [EPR10273] to HORMAD1 - C-terminal Tested applications WB Species reactivity Reacts with: Human Does not react with: Mouse, Rat Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HORMAD1 aa 350 to the C-terminus (Cysteine residue). The exact sequence is proprietary. Database link: Q86X24 Positive control HepG2 and HUVEC cell line lysates. General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR10273 Isotype IgG Applications Our Abpromise guarantee covers the use of ab178432 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. 1 Application Abreviews WB Notes 1/1000 - 1/10000. Detects a band of approximately 37-45 kDa (predicted molecular weight: 45 kDa). Application notes Is unsuitable for Flow Cyt,ICC,IHC or IP. Target Relevance HORMAD1 is a cancer/testis (CT) antigen; it is expressed strongly in testis, where it is increased in cancer and weakly in placenta. HORMAD1 shows homology to a yeast meiosis specific protein. Cellular localization Nuclear Anti-HORMAD1 antibody [EPR10273] - C-terminal images All lanes : Anti-HORMAD1 antibody [EPR10273] - C-terminal (ab178432) at 1/1000 dilution Lane 1 : HepG2 cell line lysate Lane 2 : HUVEC cell line lysate Lysates/proteins at 10 µg per lane. Secondary Goat anti-rabbit HRP conjugated antibody Western blot - Anti-HORMAD1 [EPR10273] developed using the ECL technique antibody - C-terminal (ab178432) Predicted band size : 45 kDa Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2