Product datasheet
Anti-Vimentin (phospho S56) antibody ab61802
1 References 2 Images
Overview
Product name
Anti-Vimentin (phospho S56) antibody
Description
Rabbit polyclonal to Vimentin (phospho S56)
Specificity
Detects Vimentin only when phosphorylated at serine 56.
Tested applications
ELISA, IHC-P, WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic phosphopeptide derived from human Vimentin around the phosphorylation site of
serine 56 (A-S-SP-P-G).
Positive control
Extracts from A549 cells treated with Nocodazole (1ug/ml, 16hours), human breast carcinoma
tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using
epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by
chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab61802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
1/20000.
IHC-P
1/50 - 1/100.
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Application
Abreviews
Notes
WB
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular
weight: 54 kDa).
Target
Form
Vimentin is found in connective tissue and in the cytoskeleton.
Anti-Vimentin (phospho S56) antibody images
All lanes : Anti-Vimentin (phospho S56)
antibody (ab61802) at 1/500 dilution
Lane 1 : extracts from A549 cells, treated
with Nocodazole (1ug/ml, 16hours)
Lane 2 : extracts from A549 cells, treated
with Nocodazole (1ug/ml, 16hours) with
immunizing peptide
Western blot - Vimentin (phospho S56) antibody
Predicted band size : 54 kDa
(ab61802)
Observed band size : 54 kDa
Ab61802 at 1/50 dilution staining human
breast carcinoma tissue without (left) and with
(right) immunizing peptide; paraffin
embedded.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Vimentin (phospho
S56) antibody (ab61802)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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