Product datasheet
Anti-ERCC1 antibody [4F9] ab113941
17 Images
Overview
Product name
Anti-ERCC1 antibody [4F9]
Description
Mouse monoclonal [4F9] to ERCC1
Tested applications
WB, IHC-P, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Recombinant full length Human ERCC1 protein expressed in 293T cells (NM_001983).
Positive control
HeLa, HT29, Jurkat and MCF7 cell lysates; HEK293T cell lysate transfected with pCMV6ENTRY ERCC1; Human colon adenocarcinoma, kidney, lung, lung carcinoma, ovary, ovarian
adenocarcinoma, pancreas, thyroid, endometrium adenocarcinoma, prostate, bladder and
bladder carcinoma tissues; HeLa cells and COS7 cells transiently transfected by pCMV6ENTRY ERCC1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol
Purity
Protein G purified
Purification notes
ab113941 is purified from Mouse ascites fluids by affinity chromatography.
Clonality
Monoclonal
Clone number
4F9
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab113941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/2000. Predicted molecular weight: 33 kDa.
IHC-P
1/50.
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Application
Abreviews
Notes
ICC/IF
1/100.
Flow Cyt
Use 0.1µg for 106 cells.
Target
Function
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair.
Involvement in disease
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4)
[MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset
affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the
corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures
and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes,
limbs, heart and kidney also occur.
Sequence similarities
Belongs to the ERCC1/RAD10/SWI10 family.
Cellular localization
Nucleus.
Anti-ERCC1 antibody [4F9] images
Overlay histogram showing HeLa cells
stained with ab113941 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
(ab113941, 0.1μg/1x106 cells) for 30 min at
Flow Cytometry - Anti-ERCC1 antibody [4F9]
(ab113941)
22°C. The secondary antibody used was
Alexa Fluor® 488 goat anti-mouse IgG (H&L)
(ab150113) at 1/2000 dilution for 30 min at
22°C. Isotype control antibody (black line)
was mouse IgG1 [ICIGG1] (ab91353,
1μg/1x106 cells) used under the same
conditions. Unlabelled sample (blue line) was
also used as a control. Acquisition of >5,000
events were collected using a 20mW Argon
ion laser (488nm) and 525/30 bandpass filter.
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All lanes : Anti-ERCC1 antibody [4F9]
(ab113941) at 1/2000 dilution
Lane 1 : HEK293T cell lysate, transfected
with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate, transfected
with pCMV6-ENTRY ERCC1
Lysates/proteins at 5 µg per lane.
Western blot - Anti-ERCC1 antibody [4F9]
Predicted band size : 33 kDa
(ab113941)
HEK293T cells were transfected with the
relevant cDNA for 48 hrs and lysed.
All lanes : Anti-ERCC1 antibody [4F9]
(ab113941) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : COS7 cell lysate
Western blot - Anti-ERCC1 antibody [4F9]
Lane 6 : Jurkat cell lysate
(ab113941)
Lane 7 : MDCK cell lysate
Lane 8 : PC12 cell lysate
Lane 9 : MCF7 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size : 33 kDa
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human colon
adenocarcinoma tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
3
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human kidney tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human lung tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human lung carcinoma
tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
4
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human ovary tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human ovary
adenocarcinoma tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human pancreas tissue
by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
5
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human thyroid tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human endometrium
adenocarcinoma tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human prostate tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
6
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human bladder tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/50, staining ERCC1 in
Paraffin-embedded Human bladder
carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ERCC1
antibody [4F9] (ab113941)
ab113941, at 1/100, staining ERCC1 in
COS7 cells transiently transfected by pCMV6ENTRY ERCC1, by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence Anti-ERCC1 antibody [4F9] (ab113941)
7
ab113941, at 1/100, staining ERCC1 in HeLa
cells by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence Anti-ERCC1 antibody [4F9] (ab113941)
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