Product datasheet
Anti-TMPRSS2 antibody [EPR3862] ab109131
5 Images
Overview
Product name
Anti-TMPRSS2 antibody [EPR3862]
Description
Rabbit monoclonal [EPR3862] to TMPRSS2
Tested applications
ICC/IF, WB, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
corresponding to Human TMPRSS2 aa 450-550 (C terminal).
Positive control
Human prostate tissue lysate, Human prostatic adenocarcinoma tissue
General notes
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class
antibodies. As a result of this work we are pleased to now offer this antibody in purified
format. We are in the process of updating our datasheets. The purified format is
designated ‘PUR’ on our product labels. If you have any questions regarding this
update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR3862
Isotype
IgG
Applications
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Our Abpromise guarantee covers the use of ab109131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
1/150.
WB
1/1000 - 1/5000. Detects a band of approximately 54,28 kDa (predicted
molecular weight: 54 kDa).
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing
with IHC staining protocol.
See protocols http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.
For unpurified use at 1/500 - 1/1000.
Target
Tissue specificity
Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary
gland.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.
Cellular localization
Cell membrane and Secreted. Activated by cleavage and secreted.
Anti-TMPRSS2 antibody [EPR3862] images
ab109131 staining TMPRSS2 in the HT-29
cell line by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with 4% Paraformaldehyde
permeabilized with 0.1% Triton X-100.
Samples were incubated with primary
antibody (1/150). ab150077(1/500) an Alexa
Fluor®488-conjugated Goat anti-rabbit IgG
was used as the secondary antibody. Nuclei
were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Anti-TMPRSS2 antibody [EPR3862] (ab109131)
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ab109131 staining TMPRSS2 in
Human prostrate adenocarcinoma tissue
sections by Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embedded
sections). Tissue was fixed and paraffinembedded, antigen retrieval was by heat
mediation in Tris/EDTA buffer pH9. Samples
were incubated with primary antibody
(1/1000). An undiluted HRP-conjugated
mouse anti-rabbit IgG was used as the
secondary antibody. Tissue counterstained
Immunohistochemistry (Formalin/PFA-fixed
with Hematoxylin. PBS was used in the
paraffin-embedded sections) - Anti-TMPRSS2
negative control rather than the Primary
antibody [EPR3862] (ab109131)
antibody.
All lanes : Anti-TMPRSS2 antibody
[EPR3862] (ab109131) at 1/5000 dilution
Lane 1 : Human prostrate Lysate
Lane 2 : LnCaP Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/1000 dilution
Western blot - Anti-TMPRSS2 antibody
[EPR3862] (ab109131)
Predicted band size : 54 kDa
All lanes : Anti-TMPRSS2 antibody
[EPR3862] (ab109131) at 1/1000 dilution
(unpurified)
Lane 1 : Human prostate tissue lysate
Lane 2 : Human prostate tissue lysate
Lysates/proteins at 10 µg per lane.
Western blot - TMPRSS2 antibody [EPR3862]
Predicted band size : 54 kDa
(ab109131)
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Immunohistochemical analysis of paraffinembedded Human prostatic adenocarcinoma
using ab109131, unpurified, at a dilution of
1/500.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - TMPRSS2 antibody
[EPR3862] (ab109131)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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