Product datasheet
Anti-EGF antibody [EGF-10] ab10409
1 Abreviews 1 References 1 Image
Overview
Product name
Anti-EGF antibody [EGF-10]
Description
Mouse monoclonal [EGF-10] to EGF
Specificity
Ab10409 was tested against recombinant EGF, Abcam therefore cannot guarantee it will be
suitable against endogenous EGF in WB.
Tested applications
Dot Blot, IHC-Fr, ELISA, RIA, IHC-P
Species reactivity
Reacts with: Human
Does not react with: Mouse
Immunogen
Recombinant full length protein (Human).
General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: 0.01M PBS, pH 7.4
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EGF-10
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab10409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Dot Blot
Use a concentration of 0.1 µg/ml.
IHC-Fr
1/500. PubMed: 17475821(see Abreview)
ELISA
Use at an assay dependent concentration.
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Application
Abreviews
Notes
RIA
Use at an assay dependent concentration.
IHC-P
1/500.
Target
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of
some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium
reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the
magnesium channel TRPM6.
Tissue specificity
Expressed in kidney, salivary gland, cerebrum and prostate.
Involvement in disease
Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4) [MIM:611718]; also known
as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal
hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features
include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes.
Sequence similarities
Contains 9 EGF-like domains.
Contains 9 LDL-receptor class B repeats.
Cellular localization
Membrane.
Anti-EGF antibody [EGF-10] images
ab10409 staining EGF in human normal skin
tissue sections by Immunohistochemistry
(frozen sections). Tissue was fixed with
formaldehyde. Samples were blocked with
10% serum for 30 minutes at 21°C followed
by incubation with the primary antibody at a
1/500 dilution for 30 minutes at 21°C. An
undiluted HRP-conjugated goat polyclonal
Immunohistochemistry (Frozen sections) - EGF
was used as the secondary antibody.
antibody [EGF-10] (ab10409)
Image courtesy of an anonymous Abreview.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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