Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
General notes
Anti-RIZ1 antibody
Rabbit polyclonal to RIZ1
WB
Reacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide derived from within residues 1 - 100 of Human PRDM2/Riz1. Read Abcam's proprietary immunogen policy (Peptide available as ab23566 .)
This antibody gave a positive signal in MCF7 whole cell lysate + Gal4 C-terminal tagged
PRDM2/Riz1.
The retinoblastoma protein is a target of viral oncoproteins. To explore the hypothesis that viral proteins may be structural mimics of cellular RB-binding proteins that normally mediate RB function, Buyse et al. (1995) searched cDNA libraries for RB-binding proteins. They reported the cloning of a cDNA for the zinc finger protein they called RIZ from rat and human cells. It is a 250kD nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. While the function of RIZ was not clear, its structure and pattern of expression suggested to Buyse et al. (1995) a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma.
Properties
Form
Storage instructions
Storage buffer
Purity
Primary antibody notes
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -
80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Immunogen affinity purified
The retinoblastoma protein is a target of viral oncoproteins. To explore the hypothesis that viral proteins may be structural mimics of cellular RB-binding proteins that normally mediate RB function, Buyse et al. (1995) searched cDNA libraries for RB-binding proteins. They reported the cloning of a cDNA for the zinc finger protein they called RIZ from rat and human cells. It is a 250kD nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related
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to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. While the function of RIZ was not clear, its structure and pattern of expression suggested to Buyse et al. (1995) a role in transcriptional regulation during neuronal differentiation and pathogenesis of retinoblastoma.
Polyclonal
IgG
Clonality
Isotype
Applications
Our Abpromise guarantee covers the use of ab9710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
Application notes
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa.
Is unsuitable for ChIP.
Target
Function
Tissue specificity
Sequence similarities
Cellular localization
S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-
9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophagespecific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.
Contains 8 C2H2-type zinc fingers.
Contains 1 SET domain.
Nucleus.
Anti-RIZ1 antibody images
Western blot - RIZ1 antibody (ab9710)
Anti-RIZ1 antibody (ab9710) at 1/1000 dilution
Secondary
Goat anti-Rabbit at 1/25000 dilution developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Karen Halls, University of Cambridge
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Western blot
All lanes : Anti-RIZ1 antibody (ab9710) at 1
µg/ml
Lane 1 : MCF7 cells + Gal4 C-terminal tagged PRDM2/Riz1
Lane 2 : MCF7 cells + Gal4 C-terminal tagged PRDM2/Riz1 with Human RIZ1 peptide ( ab23566 ) at 1 µg/ml
Secondary
Goat Anti-Rabbit IgG H&L (HRP) ( ab6721 ) at
1/5000 dilution
Performed under reducing conditions.
Observed band size : 47 kDa
Exposure time : 30 seconds
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