Product datasheet
Anti-TOB antibody ab168947
3 Images
Overview
Product name
Anti-TOB antibody
Description
Mouse polyclonal to TOB
Tested applications
WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Full length Human TOB (amino acids 1-345; NP_005740.1).
Run BLAST with
Positive control
Run BLAST with
HeLa cells; TOB transfected 293T cell lysate; Human endometrium tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
pH: 7.20
Constituent: 99% PBS
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab168947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.
IHC-P
Use a concentration of 3 µg/ml.
ICC/IF
Use a concentration of 10 µg/ml.
Target
1
Relevance
The Tob/Btg family of proteins consists of a large number of members. These proteins have a
common domain in their amino terminal end and may have anti-proliferative activity in various
cell types. The Tob protein was identified in a search for molecules that interact with the receptor
tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of
Tob. However, Tob is phosphorylated on serine and threonine residues but not on tyrosine,
suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob.
Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1
kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine
and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo
and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob
knockout mice develop greater bone mass resulting from increased numbers of osteoblasts.
Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein)
activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast
proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins.
Anti-TOB antibody images
All lanes : Anti-TOB antibody (ab168947) at
1 µg/ml
Lane 1 : TOB transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Western blot - Anti-TOB antibody (ab168947)
developed using the ECL technique
Predicted band size : 38 kDa
Immunohistochemical analysis of formalinfixed, paraffin-embedded Human
endometrium tissue labeling TOB with
ab168947 at 3 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - TOB antibody
(ab168947)
2
Immunofluorescent analysis of HeLa cells
labeling TOB with ab168947 at 10 µg/ml.
Immunocytochemistry/ Immunofluorescence Anti-TOB antibody (ab168947)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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