REPORT ON STATUS OF MAIZE LETHAL NECROSIS DISEASE AND GENERAL MAIZE PERFORMANCE STAKEHOLDERS’ MAIZE TOUR Dates: 2nd to 12th July, 2012 The Maize Tour Team The Maize Tour Team at Sotik DAO’s Office. From left: Sotik Staff, Dr. J. Ochieng (KARI), Dr. A. Wangai (KARI), Mr. S. Miyogo (PCPB), Mrs. T. Karanja (PPSD), Mrs. H. Oduor (PDA’s Office Rift Valley), Dr. E. Kimani (KEPHIS), Sotik staff, Sotik Staff, Dr. J. Irungu (Director, Crops Management), Dr. E. Sikinyi (STAK), Dr. Z. Kinyua (KARI), Dr. P. Ngaruiya (PCPB), Dr. D. Ligeyo (KARI) and Mr. S. Kipkemboi (DAO Sotik) ii EXECUTIVE SUMMARY Maize is widely grown in Kenya and ranks highly in food security, meeting dietary preferences of many communities in Kenya. In 2011, the area under maize production was 2,131,887 Ha; realized yields were 37.5 million bags of dry maize and 4.6 million bags of green maize. Total maize crop was worth 87.8 billions. Hence any factor that threatens maize production becomes a national food security issue In September last year, cases of maize infected by Maize Lethal Necrotic Disease (MLND) were reported in Bomet County. From January 2012 to date, the disease has been reported in other parts of the country, with implication of high yield reduction on affected farms. The MLND disease is caused by a combination of two viruses; the Sugarcane mosaic virus (SCMV) and Maize chlorotic mottle virus (MCMV) which are transmitted by aphids and thrips (Frankniella sp.) respectively. Infected maize plants show chlorotic mottle on the leaves usually starting from the base of the young leaves in the whorl and extending upward towards the leaf tips. Advance infection in the leaves show necrosis of leaf margins that progress to the midrib resulting in eventual drying up of the whole leaf. Severely infected plants show dead heart symptoms and eventual plant death. Plants that are affected late shows chlorotic mottling on the leaves and dry leaves starting from the top and form small cobs with a few or no grains. Fungal infections were also observed with whitish and pinkish growth starting from the base of the cobs. The immature cobs showed yellow streaks (cobs appears physiologically mature, but grains inside are wetwhile the rest of the plant is still green) before finally drying and rotting. A Multi Disciplinary Team (MDT) comprising both public and private sector, with varied speciality including pathologist, virologists, entomologist and maize breeder has been handling the disease. Activities undertaken include pathological surveys, lab diagnosis both locally and abroad and consultative forums. Due to the dynamic nature of this disease, the ministry sent members of the Multi Disciplinary Team (MDT) to carry out rapid assessment of the disease and verify its effect on maize performance in selected areas. The team visited Eastern, Rift Valley, Nyanza and Western provinces iii The assessment involved interacting and interviewing the District Agricultural Officers (DAO), making general observations while driving through, visiting and interviewing farmers besides collecting diseased samples for laboratory analysis. Through interaction with the farmers, the team noted that MLND has been in Kenya since June 2011 in Bomet, Naivasha and Narok. However its effect on crop planted then was considered mild. The team observed that from June 2011, the disease has since spread and has high incidence and severity in some areas, while entry of disease has been noted as recently as June 2012 in areas like Embu, Meru and Trans Nzoia. There are zones in the country which are free of disease including Bungoma, Kakamega, Machakos and Makueni. Moderate disease levels were found in upper zones of Embu North, Upper Meru central and Igembe South. The disease may not cause much havoc in this region but water stress will affect yield significantly. The farms affected by MLND in this region few, approximately 5%, and hence low overall yield reductions. High disease incidence was found in Naivasha, Bomet, Chepalungu, Sotik and Borabu Districts. In highly affected areas, yield losses of over 90% may occur and this may affect food security and livelihood in those areas. The area affected by disease out of total maize growing land in this region is high, over 80%, with high implication onfood security in the region. It was observed that there were isolated case of MLND in Nyakinyua farm in Trans Nzoia East district and Kambi Chonjo in Eldoret west district. This need to be destroyed urgently to prevent disease spread in the Kenya’s grain basket. Preliminary screening of germplasm in KARI sites in Naivasha and Bomet indicate that some tolerant lines may be identified in the long term. Unconfirmed reports from alarge scale commercial farm in Narok, Talent farm, showed that application of additional seed dressers, foliar application of insecticides, fungicide and foliar feeds has helped to reduce disease incidence and severity. The team observed overall performance of the long rains 2012 maize crop to be good, approximately the nation will realize over 85% of expected yields. In conclusion, urgent measures need to be put in place through public-private partnership to halt spread of Maize Lethal Necrotic Disease. Within a span of one year, from June 2011 to June 2012, the farmers are experiencing 100% loss in the primary MLND outbreak zones of Bomet and Naivasha districts. iv Contents EXECUTIVE SUMMARY ............................................................................................................. iii ABBREVIATIONS ..........................................................................................................................vi CHAPTER ONE: INTRODUCTION ............................................................................................... 1 1.1 National Maize Production ............................................................................................... 1 Maize production in 2011 ............................................................................................................. 1 1.2 Genesis of Maize Lethal Necrosis Disease ....................................................................... 2 CHAPTER TWO: FIELD OBSERVATIONS .................................................................................. 4 2.1 Data Collection Methodology ............................................................................................ 4 2.2 Maize Lethal Necrosis Disease situation .......................................................................... 4 2.3 Crop diversification options and challenges ..................................................................... 8 2.4 The Maize Tour Pictorial ................................................................................................ 10 2.5 Specific Maize Lethal Necrosis Disease Concerns ......................................................... 12 2.5.1 Farmers awareness on new maize disease ................................................................ 12 2.5.2 Extension staff awareness of disease: ........................................................................ 12 2.5.3 Spread of Maize Lethal Necrosis Disease (MLND) ................................................. 12 2.5.4 Effects of maize variety and crop growth stage to susceptibility to Maize Lethal Necrosis Disease (MLND) ......................................................................................................... 13 2.5.5 Effect of external factors on MLND infestation ....................................................... 13 2.5.6 Alternative Hosts to MLND ....................................................................................... 13 2.5.7 Opinions of the farmers concerning the source of the MLND .................................. 13 2.5.8 Perceived effects of MLND......................................................................................... 13 2.5.9 Role of Maize in food security and income generation ............................................ 14 2.5.10 Use of certified seeds and Fertilizers ......................................................................... 14 2.5.11 Cropping patterns in high MLND infestation .......................................................... 14 2.5.12 Diversification of farm enterprises ............................................................................ 14 2.5.13 Other Constraints in Maize farming ......................................................................... 14 2.5.14 Large-scale maize farming ......................................................................................... 15 2.5.15 KARI TRIAL SITES (Naivasha, Bomet) .................................................................. 16 2.5.16 OTHER REQUESTS MADE BY FARMERS ......................................................... 16 2.6 OBSERVATIONS ON GENERAL CROP PERFORMANCE ...................................... 16 CHAPTER THREE: RECOMMENDATIONS .............................................................................. 18 3.1 Trans Nzoia region specific recommendation ................................................................ 18 3.2 Short term Recommendations ......................................................................................... 18 3.3 Long term Recommendations.......................................................................................... 20 3.4 Conclusions and Way forward ........................................................................................ 20 CHAPTER FOUR: APPENDICES ................................................................................................. 22 4.1 Appendix 1: List of Participants...................................................................................... 22 4.2 Appendix 2: Field Programme ............................................................................................ 22 4.3 Appendix 3:DATA COLLECTION CHECKLIST ......................................................... 23 4.4 Appendix 4: Details of GPS readings and farms/sites visited ........................................ 25 4.5 Appendix 5: Altitude range of visited farms per county ................................................. 28 v ABBREVIATIONS AAK: Agrochemical Association of Kenya PCPB: Pest Control Products Board KEPHIS: Kenya Plant Health Inspectorate Services STAK: Seed Trade Association of Kenya CGA: Cereal Growers association KARI: Kenya Agricultural Research Institute NARL: National Agriculture Research Laboratories PPSD: Plant Protection Services Division MOA: Ministry of Agriculture PDA: Provincial Director of Agriculture MDT: Multi Disciplinary Team MLND: Maize Lethal Necrotic Disease FERA: Food and Environment Research Agency DAO: District Agricultural Officer Ha: Hectare MCMV: Maize Chlorotic Mottle Virus SCMV: Sugarcane mosaic virus CLN: Corn Lethal Necrosis vi CHAPTER ONE: INTRODUCTION 1.1 National Maize Production Maize is the most important staple food in Kenya. About 90 % of the Kenyan population depends on the crop directly or indirectly in terms of food, employment and income. Maize is a food security crop in the country as well as a commercial enterprise in Rift Valley and some parts of Central, Western and Eastern Provinces. Last year 2,132,000 hectares were under maize crop which is a large percentage of the total land under crop production. Maize production in 2011 Province Area (Ha) Dry maize Green Maize Total Production yields (90kg yields (90kg (90kg bag) bag) bag) Central 182,248 1,708,726 970,322 2,679,048 Rift Valley 650,270 19,196.203 1,775,132 20,971,334 Eastern 540,854 3,457,007 894,377 4,643,134 Western 255,511 5,795,028 405,990 6,201,018 Nyanza 319,483 5,864,990 308,684 6,173,674 Coast 179,499 1,482,225 303,442 1,785,666 North 3,130 8,995 Nil 8,995 2,131,887 35,520,694 4,662,960 42,183,654 Eastern Total Maize is grown in all the Provinces but the Rift Valley Province commands almost 50% of the annual production. The other key production regions include Western, Nyanza and Eastern provinces accounting for approximately 14% annual production each. Central province produces about 6% of annual total yields. Annual consumption of maize is on the increase and currently stands at approximately 38 million bags. There are many commercial cultivars of maize available to the farmers. However the number of farmers using certified seeds varies from region to region with the Rift Valley leading with adoption at 70%. Maize production is limited by several biotic and a biotic factors. Environmental conditions such as drought and soil fertility have been the major a biotic factors. Low rates of adoption of new technologies such as use of certified seeds and other agronomic practices contribute to low yields. 1 Amongst the important biotic factors affecting maize production include the infestation by weeds, insect pests especially the maize stalk borers. Diseases such as Grey leaf spot, Northern leaf blight, rusts, rots (root, stalk and ear), smuts and maize streak virus. The factors have often contributed to significant yield losses of the crop estimated at 30% annually 1.2 Genesis of Maize Lethal Necrosis Disease In September, 2011 the occurrence of an unfamiliar disease was reported in the low altitudes of Bomet District (Longisa Division, Alt 1900.masl) affecting the second season maize crop. The disease was observed to spread to the high altitudes areas of Bomet District (Bomet Central Division-Megiri, alt 2100m asl). Later the disease was noted spreading into the neighboring Chepalungu, Narok South, Narok North and Naivasha districts. By June 2012, a number of districts in South Rift had reported the disease. The infected plants in the field show a diverse range of symptoms. Diseased plants develop symptoms characteristic of virus diseases that include chlorotic mottle on the leaves, usually starting from the base of the young leaves in the whorl and extending upwards toward the leaf tips; mild to severe leaf mottling; dwarfing and premature aging of the plants; necrosis of leaf margins that progress to the mid-rib resulting in drying up of the whole leaf and necrosis of young leaves in the whorl before expansion, leading to a ‘dead heart’ symptom and plant death. In September 2011, DAO Bomet submitted samples for analysis in KARI NARL and a report was given. In January 2012 the Rift Valley Provincial Director of Agriculture sent officers in conjunction with KARI, Egerton University and KEPHIS to carry out a rapid assessment. Due to the magnitude of the disease, the PDA sought further guidance from Plant Protection Services Division (PPSD) at Ministry of Agriculture Headquarters. The head of PPSD, invited relevant institutions for a rapid field survey and followed by consultative meeting of stakeholders (Egerton University, KARI, KEPHIS, Pest Control Products Board, Twiga Chemicals, Bayer Crop Science, PPSD, Kenya Seed Ltd). During this meeting, various recommendations were made including further diseased samples analysis by KARI, Egerton University, University of Nairobi and MoA. In February/March 2012, samples collected from the infected maize fields were tested for viruses at the Kenya Agricultural Research Institute’s (KARI) National Agricultural Research Laboratories (NARL), Ohio State University (USA) and Food and Environment Research Agency (FERA), UK. The investigation revealed the presence of a new virus in Kenya, the Maize chlorotic mottle virus (MCMV) (genus Machlomovirus, Group Tombusviridae). Sugarcane mosaic virus (SCMV), a pathogen prevalent in many parts of 2 Kenya affecting cereal crops was also detected in these samples. The double infection of MCMV and SCMV or any of the cereal viruses in the Potyviridae Group (e.g. maize mosaic virus, wheat streak mosaic virus) gives rise to what is known as Maize Lethal Necrosis (MLN) disease, also referred to as Corn Lethal Necrosis (CLN) disease. The MCMV is reported to be transmitted mechanically and spread by several insect vectors, maize (corn) thrips, maize (corn) rootworms and Leaf beetles while the SCMV is spread by maize aphids. Due to many reports of suspected occurrence of this maize disease in several parts of the country, the Ministry mandated members of the Multi disciplinary team to carry out a National Maize Tour to all the important maize producing areas. The objective of the Tour was to assess the extent of the spread of the new disease and get glimpse of general performance of the long rains maize crop. The Team comprised of representatives from MoA, PCPB, KARI, KEPHIS, STAK and CGA. The Maize Tour was conducted in Rift Valley, Nyanza, Western and Eastern Provinces from 2nd to 12th July, 2012. 3 CHAPTER TWO: FIELD OBSERVATIONS 2.1 Data Collection Methodology The following methodology was used by the Multi Disciplinary Team (MDT) to asses’ disease conditions a) Administration of questionnaire to farmers b) Briefs from DAOs c) Interacting and listening to farmers and Extension staff d) Rapid field assessment by MDT e) Observations of MLND and general crop performance during movement in various regions by MDT, A check list in form of questionnaire was developed to enable the fact finding multi disciplinary team gather data giving approximate implication of MLND on resultant yields, verify factors that influence this disease and give general crop performance for long rains season 2012. Disease incidence was scored as Percentage of infected plants out of total plants. Disease severity was scored on 1-5 scale depicting level of infestation. The yield reduction percentage was based on projection of visual observation of crop, stage of growth and possibility of crop ever reaching maturity. 2.2 Maize Lethal Necrosis Disease situation Results of rapid assessment of disease incidence and severity on sampled farms, field observations made as the team drove along the districts together with briefs presented by DAOs were used to show variance of MLND across the regions as shown in Table 1. However, due to the dynamic nature of this disease and the rapid spread noted once the disease sets in, it is important to note that within a short interval of two weeks, the disease situation can have drastic implications in areas currently rated low. 4 Table1. Maize Lethal Necrosis Disease (MLND) situation in visited areas County Date of MLND MLND Expecte d yield st (districts 1 incidence severit loss (%) visited) observa (%) range y due to tion (scale MLND of 0-5) Embu: Sept 2011 60 -100 2.0 – 10 – 80 4.0 (Embu North) Meru : June 2011 60 – 100 1.5 – 4.0 October- 70 – 100 Novembe r 2011 2.0 – 4.5 Novembe 90 - 100 r 2011 3.0 – 5.0 Septemb 80 - 100 er 2011 2.5 – 4.5 (Igembe S, Igembe N, Imenti S, Meru Central) Nakuru: (Naivasha) Narok: (Narok South) Bomet: (Bomet, Chepalung u Sotik) Comments Approximately only 5% of total maize growing areas in Embu North district is affected by MLND; On the few affected farms, yield losses up to 80% have occurred; But in comparison, the affected population out of total population currently is low. However due to rapid spread, situation can drastically change. There is irrigation and continuous maize cropping in affected areas. 40 – 70 Onset and spread of MLND within Igembe South, Igembe North, Imenti South, and Meru Central districts is higher. However disease damage on crop planted in 2011 was not as severe compared to incidence on crop planted in 2012. Severe attack of MSV in Imenti South was observed. Approximated yield losses, 70% in affected regions. Further yield looses due to moisture stress in lower regions (Igembe N especially) are anticipated 60 – The small scale farmers noted disease 80 from 2011, but situation become severe from March 2012.The disease has spread to main maize growing zone of district, with observed 80% yield reduction. There is remarkable moisture stress and low soil fertility compounding the negative field situation as one drives in the district. Yield loss of up to 100% experienced in Sweet corn and Baby corn cultivars 80 – Observations made while driving in this 90 district portrays widespread infestation over large portions of the farms by MLND. The disease severity is high with death of young crop about knee high, implying 100% loss. Minimal disease incidence found in Talent Farm, with appr. 4,000 Ha under maize. 70 - 90 Within the districts of Bomet county, the disease situation is very severe on crop planted in 2012, with 100% loss in yields, crop dies while at knee high. The disease has spread throughout the entire region. 5 County Date of MLND MLND Expecte d yield st (districts 1 incidence severit loss (%) visited) observa (%) range y due to tion (scale MLND of 0-5) Kisii: February 90 - 100 4.0 – 80 – 2012 5.0 90 (Borabu) Kakamega: Not yet 0 0 0 0 0 0 (Ikolomani ) Not yet Bungoma: (Bungoma East) Trans To be Nzoia: confirme d Kitale Prison Farm Nyakinyua June Farm 2012 80-90 2.5-4.0 50-70 Eldoret North (Moi’s Bridge,Mat unda,) 5-20 3.0 2080% Machakos: Not yet 0 0 0 0 0 0 (Machakos Kathiani) Makueni: Not yet 6 Comments Affected farms in this district have high, 90% yield loss. In the area affected by disease, farmers are agitated and volatile, rigid on growing maize, rejected out rightly advice given. No symptoms of MLND were noted. Crop appearing very promising (15 – 35 bags per acre expected) MLND was not observed, but yellowing, stunting and death was noted, in localized patches, samples collected for lab analysis, suspected fungal infections Stunting and yellowing was noted on crop planted in prison farm. However only one plant expressed symptoms very close to MLND symptoms. Samples were taken for lab analysis. Slow crop growth could also be due to leaching after heavy rains in the region. Isolated pockets of severe attacks by MLND infestation was identified at Nyakinyua Farm. Reports were made by farmers of similar disease in the adjacent farms. Due to heavy population of surrounding maize crop, this primary source has potential to trigger an epidemic in the region unless urgently controlled. General crop very promising MLND observed in a farm in Kampi Chonjo. Inidence low in older crop (2%) but more intense in younger crop (30%). MSV severe, 80%. This is an isolated case surrounded by many farms with young crop that can be lost if infected by MLND. MLND was not noted in this district. However significant attacks by stalk borer were noted. The farmers are keen on dry planting, to maximize on rainfall, use of certified seeds but no control for stalk borer. Close survailance required in this area. Moisture stress may reduce yields by 20% No MLND symptoms were noted. Nutrient deficiency- phosphorus was noted to be high, stalk borer incidence 80%. However a few farmers practicing County Date of MLND MLND Expecte Comments d yield st (districts 1 incidence severit loss (%) visited) observa (%) range y due to tion (scale MLND of 0-5) GAP may get yields of 6 bags per acre. Moisture stress effect, 20% yield reduction. 7 2.3 Crop diversification options and challenges In areas where the disease has established, continuous planting of maize is a waste of resources. The MLND kills the plants very early, such that even stovers that may be harvested for animal fodder are greatly reduced. Promotion of alternative crops to break disease cycle is crucial. From field observations made by MDT and interaction with farmers, some farmers have already realized they have to adopt alternative crops to cope with the disease. However, majority are still rigid and very fearful of losing out on maize production and consumption, expressing maize meal as their main food. However, majority of farmers had other crops growing in their farms as shown in Table 2. Table 2: Crop Diversification Options County (districts visited) Embu (Embu North) Meru (Igembe S, Igembe N, Imenti S, Meru Central) Maize varieties grown H614, H6213, H627, H628, H513, H516 H614, H624, H627, H628, H513, H515, H516, DK 8053, PHB 3253, Duma H614, H627, H628, H 6210 H515, H516 DUMA Alternative Challenges crops grown Cabbages Irish potatoes Carrots Kales ; Sweet potatoes Field Beans Climbing Beans Availability of clean planting materials for potato, sweetpotato Black rot in brasicas Poor marketing infrastructure Frequent drought Exploitation by middlemen Availability of quality seeds and other planting materials Beans, Dolichos Bananas, Pawpaws, Mangoes, sorghums, millets, pigeon peas, green grams, sweet potatoes Nakuru Irish potatoes, Availability of (Naivasha) Sweet potato quality seeds, Pyrethrum Unreliable Garden Peas, rainfall Shallots Cost of farm Kales, inputs Cabbages Climbing beans, Livestock fodder (Lucerne) Narok (Narok PAN 691, Wheat, Drought, crop diseases South) Olerai 22, sunflower, Human wildlife Olerai 46, potato conflict H500 Garden peas, 8 Comments Promote commodity marketing chains Boost availability of clean planting materials New market constructed in the area under Programmes funded by GoK/NGO/Developmen t partners will boost marketing of bananas and horticultural produce Cold dry weather, low soil fertility, Low use of certified maize seed besides poor agronomic practices affect crop production Opportunity for large scale farming, and rapidly expanding populations in town and County (districts visited) Bomet (Bomet, Chepalungu, Sotik) Maize Alternative Challenges varieties crops grown grown series sorghum H614, H513 Olerai 22 Kisii (Borabu) H614, Local Var 24 Kakamega (Ikolomani) H614 & other H600 series Bungoma (Bungoma East) Trans Nzoia H614, H6213, H6210 Eldoret West Machakos: (Machakos Kathiani) Makueni: Potato, Beans, Periods of moisture Sorghum, stress Fodder for dairy animals Beans, finger millets, bananas, sweet potato Beans, bananas, sweet potato, sorghum Poor road infrastructure, rigid mentality, abuse of local brews - Comments tourist hotels offer optimum marketing channels Community had overreliance on maize as food and cash crop. Maize grown to be sold green for income. Community had overreliance on maize as food and cash crop. Appreciable level on use of fertilizers and certified seeds. Commercial maize production. Low financial capacity Potential for increased to access farm inputs maize production is high (seeds and fertilizers) Maize is main crop in this county Sugarcane, beans, finger millet Vegetables, tomatoes, beans, sunflower, sorghums Beans, millets, Soil fertility Stalk borer attack Beans, sorghums, millets Sweet potatoes, mangoes Beans, sorghums, millets Sweet potatoes, mangoes Pawpaw 9 Community expressed dietary preferences for maize meal Promote early maturing, drought tolerant crops Drought Access to affordable inputs Termites attack Stalk borer infestation Drought Promote early maturing, Access to drought tolerant crops affordable inputs Termites attack Stalk borer infestation 2.4 The Maize Tour Pictorial Public and private institutions were represented in the maize tour team that traveled to several counties and interacted closely with extension officers and farmers. The team listened to farmers and made observations in maize farms. A wide range of symptoms of maize lethal necrosis disease were observed but there was a common pattern of symptom expression, largely affecting the top leaves of the plants. 10 Maize ears were affected in some instances, resulting in poor grain set and occasional cob rotting. Farmers curiously came to listen to the team and commonly asked questions and presented memoranda petitioning the government to intervene urgently on the maize disease. Many farmers urged for help in subsidized seed for alternative crops and fertilizers. Some farmers abandoned their affected maize crops while others cut the plants for use as fodder, albeit amid other difficulties; others decided to allocate sizeable portions of their land to other crops. Poor agronomic practices and other pests lower maize production potential. 11 2.5 Specific Maize Lethal Necrosis Disease Concerns 2.5.1 Farmers awareness on new maize disease A) When the unfamiliar disease, which has since been identified as MLND, appeared in 2011 in Bomet County, farmers realized that it was not one of the usual problems but were unable to distinguish whether the condition was due to changes in weather patterns or a disease. Under the circumstances, a few farmers reported the cases to agricultural officers, local administration and agro-input dealers. The effect of the disease on crop planted in April-June, 2011 was considered to be mild. B) In 2012, farmers in many more areas in the country noticed unfamiliar symptoms on their maize crops and also received information on the disease from mass media. With increased awareness of the existence of the disease, farmers became more sensitive to any unhealthy condition on their maize crops, thereby reporting even unrelated symptoms. 2.5.2 Extension staff awareness of disease: The extension staffs are aware of the new diseases; however some said they were not able to differentiate the symptoms due to MLND from those due to moisture stress combined with premature aging of plant. The DAOs were able to access information passed via PDAs from Nairobi on MLND management. The DAOs have been passing messages on this disease during the District Commissioners open forums. The extension staffs were sensitizing farmers on recommendations enlisted within first brochure prepared in May. However, some recommendations have since been amended, and this needs quick communication to all DAOs in affected districts. The brochures prepared to guide on management of MLND need to be serialized, to enhance uptake of new changes as more studies on this disease get concluded. 2.5.3 Spread of Maize Lethal Necrosis Disease (MLND) The Maize Lethal Necrosis Disease (MLND) was noted to be prevalent in the tea growing zones in Eastern and Rift Valley provinces. From the seriously affected areas in Eastern and Rift valley regions, the maize crop is grown continuously year round. This crop is usually sold as green maize with high returns than dry maize. In the North Rift, isolated pockets of the disease were noted, largely surrounded by clean maize crop. The lower regions of Eastern Province and 12 sampled farms in Western Province were noted to be currently free of MLND. Rapid spread within and outside outbreak zones with dramatic death effects on crops were noted especially in Bomet County. 2.5.4 Effects of maize variety and crop growth stage to susceptibility to Maize Lethal Necrosis Disease (MLND) All commercial varieties planted are affected including crops from farmers’ own-saved seeds. Infestation with disease at early stages of crop growth produces severe effects on crop yields as compared to late entry which allow plant to partially escape. However, the disease was observed to attack crops at all stages of growth. The disease expression is enhanced by drought conditions 2.5.5 Effect of external factors on MLND infestation It was noted that poor crop management aggravates the disease prevalence and severity. Land degradation and low soil fertility aggravates this disease. The team noted high levels of insect pests like aphids, thrips, and bugs on infested plants. Many farmers noted that MLND is more severe during periods of moisture stress, but disease levels decrease with increased precipitation. 2.5.6 Alternative Hosts to MLND The team noted ‘suspected’ viral symptoms on possible alternative host crops like Napier and other grasses, and samples were taken for testing. 2.5.7 Opinions of the farmers concerning the source of the MLND Farmers’ views on origin of MLND include; Contaminated seeds from agro vet shops Government relief seeds Insects damage Frost damage Do not know the source God’s vengeance due to sins 2.5.8 Perceived effects of MLND The Public is scared about the likely implications of the new disease and its effects on Food Security and Farm income. Many expressed fear that suitable control measures will be identified after many years. The farmers’ 13 capacity to repay bank loans and afford schools fees in the face of expected food shortage is a major concern. In Embu- Meru region, it was noted that the main maize growing areas are not seriously affected by the disease. Food security may therefore not be adversely affected. In Naivasha, Narok, Bomet County and Borabu area of Nyamira County, it was noted that maize crop losses of up to 90% may be incurred. In these regions, maize is ranked number one both as a food security crop and for income generation. 2.5.9 Role of Maize in food security and income generation The team observed that from the sampled farmers, about 50% grow maize for income, 40% for both food and income while only 10% grow maize for subsistence. Hence any factor that will interfere with maize production will have lasting negative impact on the farming community. 2.5.10 Use of certified seeds and Fertilizers Farmers’ source certified planting seeds from local agro vets while a certain group of farmers use own saved seeds from previous seasons and also locally sourced uncertified seeds due to financial constraint. 2.5.11 Cropping patterns in high MLND infestation From sampled areas, farmers practice relay cropping due to continuous availability of moisture and have no clear cropping calendar. Over 60% of farmers’ plant pure stands and 40% practice mixed cropping. Hence in these regions, there is always a maize crop at different growth stages, implying availability of susceptible host all the time. 2.5.12 Diversification of farm enterprises Many farmers in affected regions plant maize as a commercial crop. However, other suitable crop varieties such as brasicas, legumes, potato, banana, sorghums, millets, and Napier grass are also planted. 2.5.13 Other Constraints in Maize farming Others constraints affecting maize performance included; Drought - poor distribution of rainfall results in total or partial crop failures Stalk borer infestation – infestation levels noted ranged from 60- 95%, many farmers take no control measures especially in Makueni and Machakos. 14 For sampled farmers, only 20% practice regular scouting for pests and diseases. This makes it more difficult to execute prevention measures and early eradication for new emerging pathogens before they infest and cause damage. Crops damage by wildlife was voiced by farmers that neighbor forested areas. Fungal diseases with significant damage on leaves (Chlorosis and necrosis), stem rot with lodging and molds growth on cobs were observed. Birds damage especially in instances of isolated early maturing crop were observed. Nutritional deficiency symptoms i.e. phosphorus especially in lower Eastern region were prevalent. Maize streak infestation was prevalent in Meru, Bungoma and Transnzoia. Lack of appropriate storage capacity and management of storage pests at small scale farm level. Due to inadequate storage capacity at farm level, private stores without appropriate capacity are undertaking crop storage with poor sanitation practices that may lead to high post harvest losses. Soil pests like termites, nematodes, cutworm, chaffer grubs were noted Land subdivision is affecting seed production due to lack of isolation distances. Encouragement of other crops like sugarcane and coffee on major maize growing zones is reducing hectarage for maize production. 2.5.14 Large-scale maize farming a) Sunripe Farm- Morendat (130 Ha) The farm noticed strange symptoms on baby corn crop from January 2012, but attributed the symptoms to frost. The farms capacity is 910 MT of baby corn per week, sold as component of a ready to cook mixed vegetables packs in Europe (the supermarkets cannot accept the mixed packs if one component is missing). The farm has been realizing far below the expected 7MT/ Ha out of the total 130 Ha that is under cultivation for the last 26 weeks since the disease was noticed. However, they continue to plant to sustain the export market of mixed vegetable pack. The farm has attempted to undertake control measures including application of pesticides, high crop nutrition, and irrigation but has not observed marked effect in reducing disease incidence. The MDT attributed the poor results in disease controls in this farm to the presence of maize at different stages all the year round. 15 b) Talent Farm (4,100 Ha) The disease was first noticed in March 2012, but is not spreading within the farm which is being attributed to the high standard of management. The farm undertakes routine nutritional, pests and disease management through aerial spraying. They also undertake additional seed treatment before sowing. In these two large scale farms, the management levels are high, use of quality inputs (certified seed & fertilizers), enhanced crop feeding with trace elements and undertake routine pest and disease management. But at sun ripe farm various blocks had maize crop at different stages of growth, resulting to higher significant losses due to the disease compared to Talent farm in Narok 2.5.15 KARI Trials Sites (Naivasha and Bomet) Observations made pointed to the possibility of some of the maize varieties expressing tolerance to the disease at this initial screening. There is need for screening for another season to verify the results, since this was the first season. 2.5.16 Other Requests Made by Farmers The following are other requests that the farmers made to the Government; The farmer’s across all regions appreciated Government efforts towards provision of affordable farm inputs - Seeds and fertilizers. In regions highly affected by disease, farmers requested government support in provision of subsidized alternative cropping materials, seeds, fertilizers and relief food The affected farmers expressed their fears and concerns about the impact of the disease on their livelihood and urged for urgent action by the Government to address this problem. A number of farmers expressed concern on shifting from their dietary preference of maize meal to proposed alternative crops 2.6 Some farmers are expecting Government compensation due to losses incurred Observations on General Crop Performance In general, the long rains maize crop is promising. Except in Bomet County where Maize Lethal Necrosis Diseases has caused total devastation, moistures stress is the next important factor that will cause yield reduction in Eastern Province. This fact finding team’s general assessment on likely yields to be realized putting into consideration the stage of growth and prevailing weather conditions are as follows; 16 Embu-Meru Region: 80-90% of expected yields will be realized. Machakos- Makueni region: 75-85% of expected yields Naivasha: 50- 60% of expected yields Narok: 40-50% of expected yields Bomet: 0-20% of expected yields Borabu: 60-70% of expected yields will be realized Kakamega and Bungoma region: 90-98% expected yields Trans Nzoia: 90-98% expected yields to be realized. Uasin Gishu: 80-90% expected yields to be realized The situation in Trans Nzoia and Uasin Gishu is still very dynamic. The infection is in isolated pockets which are surrounded by large fields under young maize that is vulnerable. Close surveillance is needed in these areas to assess the disease spread. 17 CHAPTER THREE: RECOMMENDATIONS The MDT made the following recommendations to enable the country cope with the current epidemic of Maize Lethal Necrosis Disease 3.1 Trans Nzoia and Eldoret North regions-Specific recommendation Isolated pockets of Maize Lethal Necrosis Disease infection was identified in these districts. In the affected farms (Nyakinyua in Trans Nzoia and Matunda in Eldoret North) disease incidence was 100% and 30% respectively. . These farms are surrounded by plantations of maize, with even seed maize plots not so far away. There is need to urgently; 1. Undertake foliar pesticide application in these hot spots and neighbouring farms to reduce the vector population, thus protect the rest of the crop 2. Destroy all affected maize stovers by burning or deep ploughing 3. Keep close surveillance on adjacent farms 3.2 Short term Recommendations a) In areas where rainfall is all year round or maize is produced under irrigation all year round there is need to harmonize the maize planting season in order to reduce disease inoculums and perpetuation. There is need to have a period where there is no maize production in the area and this may need enforcement for compliance. Each area should have defined planting period. b) Enforce local quarantine and avoid movement of stovers and green maize from affected areas to non-affected areas: Collaboration with the Local Administration for enforcement of the recommendation including removal of infected materials from the fields. c) Seed companies/ seed units to ensure that seeds are treated with appropriate seed dressers at the recommended rates. There is need to have guidance from PCPB on products range which will be used for MLND management d) There is need to give guidance on regime of MLND management including pesticide application in the field. Towards this end, the Government support is requested to support screening of pesticides e) The PCPB will through AAK give open invitations to companies to front products and support work on screening available pesticides products able to control the virus vectors. f) Diversify crops planted and practice crop rotation with non-cereal crops like beans or other legumes, onions, pumpkins, bananas, potato and sweet potato. 18 Each area should have recommended rotation programs with specific crops. This may require enforcement particularly in the major maize growing areas. g) Promote Good Agricultural Practices (fertilizer, timely weeding and control of pests and diseases) to boost plant vigor and eliminate alternate hosts for vectors. h) Support small scale farmers with subsidized registered systemic and contact pest control products for management of vectors (aphids and thrips) especially in main maize growing zones where MLND incidence is still low i) All released maize varieties should be screened for resistance to this new disease. This should be done in a coordinated manner at defined sites as in NPT. j) Support disease diagnostic laboratories at KEPHIS and KARI to boost capacity for pests and disease identification and characterization. k) Support rapid alert, emergency response and disease surveillance for early detection for prevention of disease spread in the country. l) Create awareness of the new disease through public campaigns supported by Public /private partnership to Extension staff, Farmers and stakeholders via Mass media – Local FM, Press release, Technical materials like brochures, poster, Sensitization forums like field days, workshops, shows etc. Farmers should be encouraged to report all cases of suspicious diseased plants m) Farmers whose fields are affected should get rid of the crop (minimize inoculums buildup) and plant alternative crops. n) Urgent research and screening of seed need to be done to determine if the disease is seed borne. o) 15: All MLND tolerant varieties among the on going NPT to be fast tracked for release and commercialization. p) 16: Cobs and grains from rotten plants should be destroyed by burning and not be fed to animals or consumed by human q) Government should consider supporting the farmers in the severely affected areas, Perhaps with subsidized planting materials and fertilizers but not free hand outs. r) There may be maize shortage in the most severely MLND affected and water stressed areas. There is need to prepare for additional supplies. 19 s) Constitute a recognized Secretariat which will be chaired by Ministry and a technical team (scientific matters) to address issues related to MLND. Get the TORs urgently (Action: MOA by 31 July 2012). All public communication on MLND will be verified at the Ministry Headquarters. t) The technical team will conduct rapid assessment of MLND to monitor spread and epidemiology in various regions from time to time. u) The Ministry will convene stakeholders’ consultative forums for up dates and sharing of new information v) The Ministry to set aside a fund for addressing issues related to MLND 3.3 Long term Recommendations a) Research institution to be supported to identify/breed germplasm with resistance to MLND and develop resistant varieties. b) The Government to support screening and registration of new pest control products for disease and vector control c) Training of laboratory staff and supply of appropriate laboratory equipment at KEPHIS and KARI to boost capacity for pests and disease identification and characterization. d) Support studies on how the disease was introduced so as to develop strategies to prevent similar occurrences in future. e) Enhance prevention of introduction and spread of new pests and diseases of major crops through rapid alert, response and surveillance programs for early detection. f) Training and advisory services to small scale and agro dealers should be enhanced on general management of pest and diseases. g) Tolerance to MLND should be included as criteria for release of new maize varieties. h) More studies on disease epidemiology needs to be supported for development of a suitable management strategy 3.4 Conclusions and Way forward a) Interaction with farmers revealed that Maize Lethal Necrosis Disease has been in Kenya since June 2011 in Bomet, Narok and Nakuru Counties. At the initial attack, the plants were able to escape, with mild effects on leaves and cobs. However, very severe symptoms with resultant plant death have been recorded on maize planted later in these primary outbreak zones. The disease intensity and spread has tremendously increased from June 2011 to June 2012, with resultant 100% crop loss in these primary zones. Districts highly affected includes Bomet, Sotik, Chepalungu and Borabu 20 b) The Multi-disciplinary Team observed that MLND is at initial stages of establishment in the Embu, Meru, Uasin Gichu and Trans Nzoia Counties. The percentage of affected farms from total farms in these regions is low. But in the few farms the disease was noted, 90% yield loss will be incurred. c) There are clean regions in the country where MLND was not observed including Machakos and Makueni Counties in Eastern Province, Bungoma and Kakamega Counties in Western Province d) Other factors like moisture stress and stalk borer infestation will influence yield of current long rains 2012 crop in areas like Embu, Meru, Naivasha, Machakos and Makueni. Over 80% of maize crop in all sampling areas is at silking, grain filling stages, with favorable moderate moisture levels, giving a projection of the country realizing 85% of overall expected yields. e) Propagation and spread of MLND has greatly been facilitated by continuous presence of maize at different growth in all zones where disease has been noted. However the disease is rapidly spreading to new regions in the country. The disease incidence varies from 100% in the severely affected farms to below 5% in less affected farms. f) Field observations made and interactions with farmers confirmed that all commercial seed varieties and local varieties are susceptible to MLND. The disease is affecting crop grown under small scale (moderate use of GAP) and large scale (high GAP standards) as long as there is continuous planting of maize. g) Although it’s they are the first screening trials, the KARI demonstration sites, gave some hope for a long term solution as some inbred lines and varieties have displayed some tolerance to MLND. h) To halt the spread of Maize Lethal Necrosis Disease, there is urgency in fast tracking implementation of the short and long term recommendations. Constituting a secretariat to guide and identify officers who will form a Multi Disciplinary Technical Team to consistently handle issues related to MLND to completion is crucial. 21 CHAPTER FOUR: APPENDICES 4.1 Appendix 1: List of Participants Name Dr Johnson Irungu Dr Esther Kimani Mrs Teresia Karanja Dr Paul Ngaruiya Mr. S.M. Miyogo Dr Evans Sikinyi Dr Anne Wangai Dr Z.M. Kinyua Dr Jaw Ochieng Mr. Mathew Mwarey Institution Director Crops-MOA KEPHIS MOA-PPSD PCPB PCPB STAK KARI KARI KARI Kenya Seed Additional team members in Eastern Province Mr. J.G. Ngatho PDA Eastern Mr. Felix Nduma PDAs office Mr. Peter Kiburih Monsanto (K) Ltd Mr Mululu PDAs office Mr Joash Wafula RELO Katumani Mr Peter Ragu East Africa Seeds Mr Stephen Agolla Elgon kenya Mr Francis Ndambuki Kenya Seed Additional team members in Rift valley Province Mrs. Hannah Odour PDAs office Esther Bii Kenya Seed Mr. Sammy Okite Monsanto Kenya Manish Tyagi Elgon Kenya Ltd Mr. Cyrus Mbaambu Sun ripe Farm Email Irungu_waithaka@yahoo.co.uk, ekimani@kephis.org, trzkaranja1@gmail.com, pcpboard@todays.co.ke, pcpboard@todays.co.ke, esikinyi@stak.or.ke, anne_wangai@yahoo.com, kinyuazm@gmail.com, jawochieng@kari.org, mwarey@kenyaseed.co.ke Telephone 0722325917 0722226239 0722267909 0722894138 0723159475 0710575207 077799879 Pda_eastern@yahoo.com Pda_eastern@yahoo.com Peter.kiburih@monsanto.com mululud@yahoo.com relokatumani@yahoo.com ragupeter@yahoo.com Stephen@elgonkenya.com ndambuki@kenyaseed.co.ke 0721940205 0721751092 0714606052 0722968844 0725890960 0722630084 0724711502 0733793415 pdarvp@yahoo.com esther@kenyaseed.co.ke Sammy.okite@monsato.com 0722726357 Additional team members in Nyanza Province Mr Owiro J.O PDA Nyanza 4.2 Appendix 2: Field Programme Day - Date Leave Arrive Monday – 2ND July Nairobi Embu Tuesday – 3RD July Embu Meru Wednesday – 4th July Meru-Embu Naivasha Wednesday – 5th July Naivasha Two farms Thursday – 6th July Naivasha Bomet Friday – 7th July Chepalungu Sotik, Borabu th Saturday – 8 July Kaka mega Sunday – 9th July Monday – 10th July Tuesday Wednesday – 11th July Trans nzoia Trans Nzoia Eldoret Makueni Thursday KARI KATUMANI Eldoret Nairobi Machakos, Kathiani 0707222526 0722614639 0714606053 0733191512 0738900274 0711827785 0722485864 Activity of the day Visit farms around Embu area Visit farms around Meru area Spend night in Naivasha Visit 2 farms around Naivasha area Visit 3 farms in Bomet county Visit farms around Sotik, Borabu Visit farms around Kaka mega and Bungoma Spend night in Kitale Visit 2 farms Machakos/Makueni areas Conclude field trip Report writing 22 No. Days 1 2 0 1 2 1 2 0 1 4.3 Appendix 3:DATA COLLECTION CHECKLIST Name of farmer: Gender: Telephone No. No QUESTION 1 Do you grow maize for a)Food b) Income c) Both 2 During the last 3 seasons which maize variety did you plant and what was the approximate hectare for each variety 3 Where do you get planting seeds and technical advice on varieties to grow 4 How do you grow your maize crop a) Mixed cropping; Mono cropping, or both District: GPS coordinates: Date: RESPONSES a) b) b) Seasonal; Continuous; Relay c) which crop preceded the current maize stand 5 6 7 What major problems have you encountered as you grow maize in your farm in the last 5 years Do you practice regular scouting for pests and diseases When did farmer ( month/year) notice the new maize disease a) At which stage was the crop What do you think may have brought this disease to your farm b) Did you notice insect pests and how was their population per plant c) Which varieties are affected on your farm d) How widespread is this disease in this geographical region 8 Which weather conditions were prevailing when disease was first noted, do you think weather is influencing this disease 9 For how many seasons has your maize been affected by this disease 10 In comparison to when this disease had not come, can the farmer approximate % yield reduction experienced on his farm due to MLND. 11 What coping strategies has the farmer undertaken to manage this disease 12 13 Has the farmer sprayed any pesticides on the maize crop Name pesticide sprayed and farmer’s observed effects of spray in management of MLND 14 Has the farmer been sensitized on this new disease a) What was the source of the information b) Is the farmer aware of recommended control measures? Is the farmer comfortable/ Not comfortable with the following a) planting maize only one season per year 15 b) Rouging and burning all infected crop materials c) Practicing crop rotation d) Planting only certified seeds e) Avoiding movement of plant materials out of their farms and also out of their 23 a) b) Name of farmer: Gender: Telephone No. geographical region f) District: GPS coordinates: Date: Controlling insects pests on maize crop 16 Which other crops or plants have been observed to be affected by this new maize disease in this region 17 If MLND makes it difficult for farmers to grow maize, can the farmer name at least 5 in order of priority alternative crops that he can plant 18 Is the farmer already growing any of the above crops? a) How is the field performance b) How is the market value c) Are the planting materials/ seeds available locally d) Do these crops meet cultural and dietary preferences of the community e) Has the farmer experienced any problem when growing any of these crops In the opinion of the farmer what roles should the following play to help manage MLND 19 a) Farmers b) NGOs c) Government 20 CROP MANAGEMENT ASPECTS a) Which type of fertilizer did the farmer use at planting and what was the source b) Which type of fertilizer did the farmer use to top dress the maize and what was the source c) How many times does the farmer weed maize crop d) At what stage of crop growth is weeding done 21 22 Soon after germination Knee height Tassel ling Opportunity for farmer to ask any questions on MLND or food security in general to the team: The team will seek permission from farmer to a) assess current MLND incidence per maize variety in the farm b) assess current MLND severity per maize variety in the farm 23 The Team will make overall comments on effects of MLND on the crop 24 a) b) 4.4 County (districts visited) Embu: (Embu North) Appendix 4: Details of GPS readings and farms/sites visited Farmer’s name Area name Altitude Latitude (m asl) range Longitude Kagumori village, Manyatta Division 1818 S00º 22.226’ E037º 26.948’ Kagumori village, Manyatta Division 1820 S00 22.212’ E037 26.955’ Manyatta Division 1898 S00 21.598’ E037 26.549’ Imenti South 1643 S00 03.456’ E037 37.960’ 1926 S00 00.290’ E037 35.371’ Severe MLND symptoms 1928 S00 00.233’ E037 35.258 Severe MLND symptoms 1006 S00 01.076’ E037 48.548’ No symptoms of MLND seen; crop very good-looking 1440 S00 11.507’ E037 55.944’ Very severe MLND symptoms 1867 S00 40.072’ E036 22.490’ 1867 S00 39.161’ E036 22.553’ Very severe MLND symptoms on baby corn 1890 S00 39.916’ E036 23.383’ Joseph Muchu Gituamba, Naivasha 2050 Central Division Tabitha Kuria Maraigushu, Kinungi, 2281 (0725-324049) Naivasha Central Division Talent Farm Narok South District 1973 (c/o Gavin, Farm Manager – 0713460740) S00 44.044’ E036 38.300’ S00 45.662’ E036 31.075’ Variations observed in MLND severity among screening entries Very severe MLND symptoms Severe MLND symptoms S01 00.596’ E035 32.468’ Lawrence Njeru Kathuri (0718-540528) Onesmus Kariuki (neighbour to Kathuri) David Nyaga Ngoroi Meru: Elijah Gikunda (Igembe S, (0788-102587) Igembe N, Imenti S, Meru Central) Jennifer Gichuru Githongo, Abothuguchi West Division, Meru Central Stella Kirigia Githongo, Abothuguchi West Division, Meru Central Peter Kaburu Gakori village, (0720-365908) Nkandone s/location, Abothuguchi East Division Stanley Kiohura village, Mugambi Igembe South District (0733-221240) Nakuru: Silas Mbaabu Sunripe Farm, (Naivasha) (Farm Naivasha manager, Sunripe Farm) Silas Mbaabu Sunripe Farm, (Farm Naivasha manager, Sunripe Farm) KARI/CIMMYT Sunripe Farm, trial site Naivasha Narok: (Narok South) 25 Altitudes Comments on range (m disease situation asl) 1818-1898 Phaeosphaeria leaf spot very severe; ear rots evident Symptoms of maize lethal necrosis disease (MLND) evident MLND symptoms evident 1006 MLND symptoms 1928 evident 1867 2281 1973 1997 Baby corn crop affected by MLND Reported to have a very low incidence of MLND; spraying with insecticides and fungicides County (districts visited) Farmer’s name Area name Altitude Latitude (m asl) range Longitude Bomet ATC, Bomet 1868 S00 47.771’ E035 20.439’ Nyambugo s/location, 1906 Sigor Division, Chepalungu District Aroiywut village, 1788 Sigor Location, Sigor Division Paul Tanui Mutarakwa Division, 1814 (0729-018306) Sotik District Lucy Moraa Borabu (roadside 1853 Andara stop over) S00 51.996’ E035 19.368’ S00 55.686’ E035 15.442’ Very severe MLND symptoms S00 45.764’ E035 13.860’ S00 43.354’ E035 02.299’ S00 46.875 E035 02.596 N00 17.216’ E034 45.377’ 1505 1549 1627 N00 42.320’ E034 44.863’ 1627 1692 Grace Nanjala Nasusi B village, 1692 Wafula (0707- Kamusinde location, 457312) Kamukuywa Division, Kimilili District N00 49.114’ E034 45.264’ Kitale Prison GK Medium Prison Farm c/o Mr. Kitale Farm, Trans Okwayo, Farm Nzoia West District Manager (0725-730643) 1865 N01 01.239’ E034 59.919’ MLND symptoms evident Very severe MLND symptoms; field clearing in progress and crop residue left in the field; crop at tasseling stage Very severe MLND symptoms; crop mature/advanced No MLND symptoms seen; crop very good and at soft dough stage Patches of maize field with chlorotic, stunted plants (root system trimming evident); suspected symptoms of MLND (samples taken) Patches of maize crop field with rotting root system and lower stem (samples taken) Only one plant with suspected MLND symptoms seen Thomas Three Rivers Farm, 1738 Wafula (0729- Kipyoywan s/location, 741625) Kinyoro location, Saboti Division Trans Nzoia West District Gerald Nyakinywa, near 1776 Wambugu (c/o Bata Muhiu Primary Kihenjo 0727- School, Trans Nzoia 167614) East District Uasin Gishu Samuel Birgen Sikaon village (Kambi 1852 N00 58.587’ E034 54.545’ N00 54.613’ E035 07.135’ N00 50.033 E035 10.449 Bomet: KARI/CIMMYT (Bomet, trial site Chepalungu , Sotik) John Cheruiyot (0720-815648) Mike Ruto Kisii (Borabu) Samson Mainye (0721621365) Kakamega Road side (Ikolomani) stop over Bungoma (Bungoma East) Trans Nzoia (Trans Nzoia West, Trans Nzoia East) Kineni sub-location, 1793 Nyansiongo location, Borabu Division Kakamega town 1549 outskirts John Upendo village, Wepukhulu Misikhu s/location, (0719-584226) Webuye Division 26 Altitudes Comments on range (m disease situation asl) done 1788 Variations 1906 observed in MLND severity among screening entries Very severe MLND symptoms 1793 1853 1738 – 1865 Crop affected by MLND but farmer got some harvest; neighbours’ crops affected Severe MLND symptoms observed 1852 Mild symptoms of County (districts visited) (Eldoret West) Farmer’s name Area name Altitude Latitude (m asl) range Longitude Altitudes Comments on range (m disease situation asl) MLND observed arap Sambu Makueni Hon. Gideon Ndambuki’s farm Chonjo), Matunda s/location, Soy Division Mukuyuni, Kaiti Division 1297 S01 45.219’ E037 27.873’ 1280 – 1330 Francis Mukuyuni, Kaiti Kyongo (0713- Division 777736) 1280 S01 44.770 E037 28.104 Regina Mukuyuni, Kaiti Mwania (0727- Division 015313) 1330 S01 44.721 E037 26.434 Luka Muthoka Kimutwa location, Central Division, (Machakos, Machakos District Kathiani) 1568 S01 34.781’ E037 16.859 Samuel Mweu Mang’auni village, (0722-335682) Kaathi s/location, Kimutwa location, Machakos District 1546 S01 33.834’ E037 16.346’ Mr. Kilonzo Mitamboni village, Mitamboni location, Kathiani District 1516 S01 23.431’ E037 16.024 Philip Mutua Kinyau s/location, Ndolo (0727- Mitamboni location, 206092) Kathiani District 1495 S01 20.414’ E037 15.256 Machakos 27 1495 – 1568 No symptoms of MLND observed; some portions of the crop affected by moisture stress No symptoms of MLND observed; very good water trapping done No symptoms of MLND observed; Good crop of Pannar 4M19 but a portion with recycled Pioneer seed performing poorly. No incidence of MLND observed; farmer pre-soaks seed to hasten germination due to fear of shortage of rains No incidence of MLND observed; farmer uses own selected seed of a local variety; applies manure and DAP but no topdressing. No incidence of MLND observed; stalk borer incidence noted No incidence of MLND observed; farmer with good agronomic practices 4.5 Appendix 5: Altitude range of visited farms per county County (districts visited) Embu (Embu North) Meru (Igembe S, Igembe N, Imenti S, Meru Central) Nakuru (Naivasha) Narok (Narok South) Bomet (Bomet, Chepalungu, Sotik) Kisii (Borabu) Kakamega (Ikolomani) Bungoma (Bungoma East) Trans Nzoia (Trans Nzoia West, Trans Nzoia East) Makueni Machakos (Machakos, Kathiani) Altitudes range (m asl) 1818-1898 1006 - 1928 1867 - 2281 1973 - 1997 1788 - 1906 1793 - 1853 1505 - 1549 1627 - 1692 1738 – 1865 1280 – 1330 1495 – 1568 28