Product datasheet
Anti-MMP7 antibody ab5706
2 Abreviews 9 References 2 Images
Overview
Product name
Anti-MMP7 antibody
Description
Rabbit polyclonal to MMP7
Specificity
This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10
or 16.
Tested applications
WB, ELISA, IP, IHC-Fr, ICC/IF, IHC-P
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen
Synthetic peptide corresponding to C-Terminus of MMP7 (Human).
Positive control
PMA stimulated A431 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Proclin
Constituents: PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab5706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
ELISA
IP
IHC-Fr
1
Application
Abreviews
Notes
ICC/IF
IHC-P
Application notes
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 18499699).
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.
IP: Use 3-5µg for 106 cells.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus
inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activationpeptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Anti-MMP7 antibody images
The whole cell lysate derived from PMAstimulated A431 was probed with rabbit anti
MMP7 at 1/500 (Lane A) or by pre-absorption
antibody with immunizing peptides (Lane B).
Western blot - MMP7 antibody (ab5706)
2
ab5706 (2µg/ml) staining MMP7 in human
kidney cortex using an automated system
(DAKO Autostainer Plus). Using this protocol
there is staining of the cytoplasm and nuclei
within proximal convoluted tubules.
Sections were rehydrated and antigen
retrieved with the Dako 3 in 1 AR buffers
EDTA pH 9.0 in a DAKO PT link. Slides were
peroxidase blocked in 3% H2O2 in methanol
Immunohistochemistry (Formalin/PFA-fixed
for 10 mins. They were then blocked with
paraffin-embedded sections)-MMP7
Dako Protein block for 10 minutes (containing
antibody(ab5706)
casein 0.25% in PBS) then incubated with
primary antibody for 20 min and detected with
Dako envision flex amplification kit for 30
minutes. Colorimetric detection was
completed with Diaminobenzidine for 5
minutes. Slides were counterstained with
Haematoxylin and coverslipped under
DePeX. Please note that, for manual staining,
optimization of primary antibody
concentration and incubation time is
recommended. Signal amplification may be
required.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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