Product datasheet Anti-p18 INK4c antibody ab53035 1 Image Overview Product name Anti-p18 INK4c antibody Description Rabbit polyclonal to p18 INK4c Specificity Detects endogenous levels of total p18 INK protein Tested applications WB, ELISA Species reactivity Reacts with: Human Predicted to work with: Mouse, Rat Immunogen Synthetic peptide derived from human p18 INK4c Positive control Cos7 cell extract Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. Storage buffer Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Purity Immunogen affinity purified Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab53035 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). ELISA 1/5000. Target 1 Function Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB. Tissue specificity Highest levels found in skeletal muscle. Also found in pancreas and heart. Sequence similarities Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. Contains 4 ANK repeats. Anti-p18 INK4c antibody images All lanes : Anti-p18 INK4c antibody (ab53035) at 1/500 dilution Lane 1 : Cos7 cell extract Lane 2 : Cos7 cell extract with peptide Predicted band size : 18 kDa Observed band size : 18 kDa Western blot - p18 INK4c antibody (ab53035) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2