Product datasheet
Anti-NORE1 antibody ab22770
3 Images
Overview
Product name
Anti-NORE1 antibody
Description
Rabbit polyclonal to NORE1
Specificity
ab22770 primarily detects NORE1 protein (isoform A) at ~50kDa in human HEK293 cells and
also in mouse brain lysate. This antibody is also expected to detect a number of NORE1
isoforms based on an immunogen BLAST search. In human HEK293 cells, ab22770 faintly
detects the following NORE1, RASSF3 and RASSF5 isoforms (Swiss Prot ID in brackets):
44kDa (Q5SY32), 39kDa (Q59GG4), 37kDa (Q8WWV9), 31kDa (Q8WXF4) and 21kDa
(Q8TEK8) bands. In mouse brain ab22770 also faintly detects NORE1 isoform B at ~32kDa
band (Q8C2E8).
Tested applications
ICC/IF, IHC-P, WB
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of
Human NORE1. Read Abcam's proprietary immunogen policy (Peptide available as ab23380.)
Positive control
HEK293 cell lysate, Mouse brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab22770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa
(predicted molecular weight: 47 kDa).
Target
Function
Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A
activation upon T cell receptor or chemokine stimulation to integrin activation. Isoform 2
stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an
enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of
microtubule growth. The association of isoform 2 with activated RAP1A is required for
directional movement of endothelial cells during wound healing. May be involved in regulation of
Ras apoptotic function. The RASSF5-STK4 complex may mediate HRAS1 and KRAS induced
apoptosis.
Tissue specificity
Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors.
Sequence similarities
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-associating domain.
Contains 1 SARAH domain.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Isoform 2 is mainly located in the perinuclear region of
unstimulated primary T cells. Upon stimulation translocates to the leading edge and colocalizes
with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to
growing microtubules in vascular endothelial cells and is dissociated from microtubules by
activated RAP1A.
Anti-NORE1 antibody images
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All lanes : Anti-NORE1 antibody (ab22770)
at 1 µg/ml
Lane 1 : HEK293 cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : HEK293 cell lysate with Human
NORE1 peptide (ab23380) at 1 µg/ml
Lane 4 : Mouse brain lysate with Human
NORE1 peptide (ab23380) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Western blot - NORE1 antibody (ab22770)
Secondary
Alexa fluor goat polyclonal to rabbit IgG at
1/10000 dilution
Predicted band size : 47 kDa
Observed band size : ~50 kDa
ICC/IF image of ab22770 stained human
Hek293 cells. The cells were methanol fixed
(5 min), permabilised in 0.1% PBS-Tween
(20 min) and incubated with the antibody
(ab22770, 1µg/ml) for 1h at room
temperature. 1%BSA / 10% normal goat
serum / 0.3M glycine was used to block nonspecific protein-protein interactions. The
secondary antibody (green) was Alexa Fluor®
488 goat anti-rabbit IgG (H+L) used at a
1/1000 dilution for 1h. Alexa Fluor® 594 WGA
Immunocytochemistry/ Immunofluorescence -
was used to label plasma membranes (red).
NORE1 antibody (ab22770)
DAPI was used to stain the cell nuclei (blue).
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IHC image of ab22770 staining NORE1 in
Human Tonsil formalin fixed paraffin
embedded tissue section, performed on a
Leica BondTM system using the standard
protocol F. The section was pre-treated using
heat mediated antigen retrieval with EDTA
(pH9, epitope retrieval solution 2) for 20 mins.
The section was then incubated with
ab22770, 5µg/ml, for 15 mins at room
Immunohistochemistry (Formalin/PFA-fixed
temperature and detected using an HRP
paraffin-embedded sections) - Anti-NORE1
conjugated compact polymer system. DAB
antibody (ab22770)
was used as the chromogen. The section was
then counterstained with haematoxylin and
mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
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