Product datasheet
Anti-FGFR4 antibody [5B5] ab44971
14 Abreviews 6 References 4 Images
Overview
Product name
Anti-FGFR4 antibody [5B5]
Description
Mouse monoclonal [5B5] to FGFR4
Specificity
ab44971 detects fibroblasts.
Tested applications
IHC-Fr, Flow Cyt, ICC, ICC/IF, IHC-P
Species reactivity
Reacts with: Mouse, Human
Immunogen
Fusion protein corresponding to Human FGFR4.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS
Purity
Protein G purified
Clonality
Monoclonal
Clone number
5B5
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab44971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-Fr
Abreviews
Notes
1/50.
1
Application
Abreviews
Flow Cyt
Notes
1/50. See Abreview.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with
this antibody.
ICC
Use at an assay dependent concentration.
ICC/IF
1/50.
IHC-P
1/30.
Target
Function
Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor.
Binds FGF19.
Tissue specificity
Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell
lines.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor
receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
Post-translational
modifications
Glycosylated.
Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a
functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization.
Cellular localization
Membrane. Isoform 2 may be secreted.
Anti-FGFR4 antibody [5B5] images
ab44971 (1/50) detecting fibroblast cells
(red). Human BJ fibroblast cells were fixed in
formaldehyde, permeabilized in 0.1% Triton
and blocked in 8% BSA.
Immunocytochemistry/ Immunofluorescence Anti-FGFR4 antibody [5B5] (ab44971)
This image is courtesy of an anonymous Abreview
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ab44971 Mouse monoclonal [5B5] to FGFR4
staining fibroblasts in normal
human skin tissue section by
Immunohistochemistry (Frozen sections).
Tissue underwent fixation in
paraformaldehyde and blocked in 10% BSA
for 30 minutes at 21°C. The primary antibody
Immunohistochemistry (Frozen sections) - AntiFGFR4 antibody [5B5] (ab44971)
was diluted 1/50 in PBT (PBS + 0.5% Tween
20 + 0.5% BSA) and incubated with sample
for 30 minutes at 21°C. A HRP
conjugated goat polyclonal to mouse IgG was
used undiluted as secondary.
ab44971 staining Human primary adult
fibroblasts by Flow Cytometry. Cells were
harvested with 1 min 0.05% trypsin treatment
at 37°C in 5% FBS. Cells were fixed with
paraformaldehyde and permeabilized with
Triton X-100. The sample was incubated with
the primary antibody, (1/50 in 5% FBS), for 20
minutes at 21°C. An Alexa Fluor®647conjugated Goat polyclonal to mouse IgG
Flow Cytometry - Anti-FGFR4 antibody [5B5]
(1/400) was used as secondary antibody.
(ab44971)
This image is courtesy of an anonymous Abreview
ab44971 Mouse monoclonal [5B5] to FGFR4
staining fibroblasts in murine abdominal
tissue by Immunohistochemistry
(Formalin/PFA-fixed paraffin-embedded
sections).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-FGFR4
antibody [5B5] (ab44971)
Image from Liao IC et al, Biomaterials. 2011
Feb;32(6):1669-77. Epub 2010 Nov 16, Fig 3. DOI
10.1016/j.biomaterials.2010.10.049
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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