Product datasheet
Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
ab134043
2 Images
Overview
Product name
Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
Description
Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)
Specificity
Only detects FGFR1 alpha when phosphorylated at Tyrosine 654
Tested applications
WB
Species reactivity
Reacts with: Mouse
Immunogen
A phospho specific peptide corresponding to residues surrounding Tyrosine 654 of Human
FGFR1 alpha.
Positive control
Constitutively active FGFR1
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react
with these species. Please contact us for more information.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 1.60 x 10 -10 M
Learn more about KD
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP843(2)
1
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab134043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Application notes
1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
Relevance
FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor
family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple
isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms
expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis
and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with
craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic
fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with Ncadherin and NCAM.
Cellular localization
Cell Membrane
Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] images
All lanes : Anti-FGFR1 alpha (phospho
Y654) antibody [EP843(2)] (ab134043) at
1/1000 dilution
Lane 1 : Control
Lane 2 : Constitutively active FGFR1 alpha
Lane 3 : Kinase dead lysates from Xenopus
oocytes
Western blot - Anti-FGFR1 alpha (phospho Y654)
Lysates/proteins at 10 µg per lane.
antibody [EP843(2)] (ab134043)
Secondary
HRP labelled goat anti- rabbit at 1/2000
dilution
Predicted band size : 130 kDa
2
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-FGFR1 alpha (phospho Y654) antibody
[EP843(2)](ab134043)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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