Product datasheet
Anti-CCN1 antibody ab24448
7 Abreviews 9 References 3 Images
Overview
Product name
Anti-CCN1 antibody
Description
Rabbit polyclonal to CCN1
Tested applications
ICC/IF, IHC-FoFr, IHC-P, IHC-Fr, WB
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Immunogen
Synthetic peptide corresponding to Mouse CCN1. Synthetic peptide surrounding amino acid
166 of mouse Cyr61. The peptide was 10-15 amino acids long.
(Peptide available as ab52125)
Positive control
Jurkat cell lysate, mouse small intestine lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab24448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
1/50. PubMed: 19077055
IHC-FoFr
Use at an assay dependent concentration. PMID 17724216
IHC-P
Use a concentration of 2 µg/ml.
IHC-Fr
Use at an assay dependent concentration. PubMed: 21490242
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Application
Abreviews
WB
Notes
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa
(predicted molecular weight: 42 kDa).Can be blocked with CCN1 peptide
(ab52125).
Target
Function
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role
in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes
involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C,
MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene
regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and
alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through
integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation
through integrin alpha-v/beta-3.
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain.
Cellular localization
Secreted.
Anti-CCN1 antibody images
All lanes : Anti-CCN1 antibody (ab24448) at
1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse small intestine tissue lysate
Lysates/proteins at 30 µg per lane.
Western blot - CCN1 antibody (ab24448)
Predicted band size : 42 kDa
Observed band size : 42 kDa
2
ab24448 staining Human myometrium.
Staining can be observed in the cytoplasm
and nuclear compartments.
Left panel: with primary antibody at 2ug/ml.
Right panel: isotype control.
Immunohistochemistry (Formalin/PFA-fixed
Sections were stained using an automated
paraffin-embedded sections)-CCN1
system DAKO Autostainer Plus , at room
antibody(ab24448)
temperature: sections were rehydrated and
antigen retrieved with the Dako 3 in 1 AR
buffers EDTA pH 9.0 in a DAKO PT Link.
Slides were peroxidase blocked in 3% H2O2
in methanol for 10 mins. They were then
blocked with Dako Protein block for 10
minutes (containing casein 0.25% in PBS)
then incubated with primary antibody for 20
min and detected with Dako envision flex
amplification kit for 30 minutes. Colorimetric
detection was completed with
Diaminobenzidine for 5 minutes. Slides were
counterstained with Haematoxylin and
coverslipped under DePeX. Please note that
for manual staining we recommend to
optimize the primary antibody concentration
and incubation time (overnight incubation),
and amplification may be required.
ICC/IF image of ab24448 stained HeLa cells.
The cells were 4% PFA fixed (10 min) and
then incubated in 1%BSA / 10% normal goat
serum / 0.3M glycine in 0.1% PBS-Tween for
1h to permeabilise the cells and block nonspecific protein-protein interactions. The cells
were then incubated with the antibody
(ab24448, 1µg/ml) overnight at +4°C. The
secondary antibody (green) was Alexa Fluor®
488 goat anti-rabbit IgG (H+L) used at a
Immunocytochemistry/ Immunofluorescence-
1/1000 dilution for 1h. Alexa Fluor® 594 WGA
CCN1 antibody(ab24448)
was used to label plasma membranes (red) at
a 1/200 dilution for 1h. DAPI was used to
stain the cell nuclei (blue) at a concentration of
1.43µM.
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