Product datasheet
Anti-TRAIL antibody [2E5] ab2219
4 References 1 Image
Overview
Product name
Anti-TRAIL antibody [2E5]
Description
Mouse monoclonal [2E5] to TRAIL
Specificity
This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing
ligand (TRAIL/ APO2-L)).
Tested applications
Functional Studies, Flow Cyt, Neutralising
Species reactivity
Reacts with: Human
Does not react with: Mouse
Immunogen
Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.4
Purity
>95% by SDS-PAGE
Purification notes
Purified from ascites using precipitation methods.
Clonality
Monoclonal
Clone number
2E5
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Applications
Our Abpromise guarantee covers the use of ab2219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Functional Studies
Abreviews
Notes
Use at an assay dependent concentration. The antibody 2E5 has high
neutralizing activity for human TRAIL in biological assays.
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Application
Abreviews
Notes
Flow Cyt
Use a concentration of 1 - 10 µg/ml.
Neutralising
Use at an assay dependent concentration. The antibody 2E5 has high
neutralizing activity for human TRAIL in biological assays.
Target
Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3,
TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity
may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3,
TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Tissue specificity
Widespread; most predominant in spleen, lung and prostate.
Sequence similarities
Belongs to the tumor necrosis factor family.
Cellular localization
Membrane.
Anti-TRAIL antibody [2E5] images
Apoptosis induced in JURKAT human T cell
leukemia cell line by soluble recombinant
human TRAIL is completely blocked by antihuman TRAIL (2E5).
The neutralizing activity of the antibody 2E5
has been confirmed with various sources of
Functional Studies - Anti-TRAIL antibody [2E5]
(ab2219)
soluble recombinant human TRAIL.
A - medium
B - recombinant TRAIL
C - recombinant TRAIL + anti-human TRAIL
(2E5; 0.06 µg/ml)
D - recombinant TRAIL + anti-human TRAIL
(2E5; 0.24 µg/ml)
E - recombinant TRAIL + Isotype mouse IgG1
control
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
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