Product datasheet
Anti-FLASH antibody ab1146
Overview
Product name
Anti-FLASH antibody
Description
Rabbit polyclonal to FLASH
Specificity
Reacts with the N terminus of the FLASH protein.
Tested applications
WB
Species reactivity
Reacts with: Mouse
Immunogen
N-terminus of the FLASH protein (proprietary peptide).
General notes
Fas is a cell-surface receptor molecule that relays apoptotic signals into cells. When Fas is
activated by the binding of its ligand, the proteolytic protein caspase-8 is recruited to a signaling
complex known as DISC by binding to a Fas-associated adapter protein. A new protein,
FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the
Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a
death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of
the DISC signaling complex and is necessary for the activation of caspase-8 in Fas-mediated
apoptosis.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
PBS with 0.02% sodium azide
Purity
IgG fraction
Primary antibody notes
Fas is a cell-surface receptor molecule that relays apoptotic signals into cells. When Fas is
activated by the binding of its ligand, the proteolytic protein caspase-8 is recruited to a signaling
complex known as DISC by binding to a Fas-associated adapter protein. A new protein,
FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the
Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a
death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of
the DISC signaling complex and is necessary for the activation of caspase-8 in Fas-mediated
apoptosis.
Clonality
Polyclonal
Isotype
IgG
1
Light chain type
unknown
Applications
Our Abpromise guarantee covers the use of ab1146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/500 - 1/1000.
Target
Function
Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at
the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses
both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alphainduced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream
mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in
FAS-mediated apoptosis. Required for histone gene transcription and progression through S
phase.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus. Localizes to discrete nuclear foci.
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