Product datasheet
Anti-FEM 1B antibody ab3686
3 Images
Overview
Product name
Anti-FEM 1B antibody
Description
Rabbit polyclonal to FEM 1B
Tested applications
WB, ICC/IF, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide:
DINYQDQIPRTLEE
, corresponding to amino acids 609-622 of Human FEM 1B(Peptide available as ab6098.)
Run BLAST with
General notes
Run BLAST with
Stable for one year.
Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death
receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1
was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the
homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3
and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1.
Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death
receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human
and mouse tissues.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Purity
Immunogen affinity purified
Primary antibody notes
Fas and Tumor Necrosis Factor Receptor 1 (TNFR 1) are two prototype members in the death
receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR 1
was recently identified and designated F1A alpha and FEM 1 beta. F1Aa/FEM1b is the
homologue of C. elegans sex determining protein FEM 1. FEM 1/F1Aa is cleaved by CED-3
and caspase. FEM 1/F1Aa associates with CED-4 and its mammalian homologue Apaf 1.
Overexpression of F1Aa induces apoptosis. F1Aa is therefore a novel member of the death
receptor associated protein that mediates apoptosis. F1Aa is expressed in a variety of human
and mouse tissues.
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Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab3686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 70 kDa.Can be
blocked with FEM 1B peptide (ab6098).
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
Target
Function
Component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate
recognition subunit. Involved in apoptosis by acting as a death receptor-associated protein that
mediates apoptosis. Also involved in glucose homeostasis in pancreatic islet. Functions as an
adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.
Tissue specificity
Widely expressed. Highly expressed in testis. Weakly expressed in other tissues.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the fem-1 family.
Contains 8 ANK repeats.
Contains 1 TPR repeat.
Cellular localization
Cytoplasm. Nucleus. In the nucleus, the protein level increased slightly after camptothecin (CPT)
treatment. Associated with chromatin.
Anti-FEM 1B antibody images
Predicted band size : 70 kDa
Western blot analysis of FEM 1B in mouse
Western blot - FEM 1B antibody (ab3686)
(A) and rat (B) liver tissue lysates with antiFEM 1B (ab3686) at 1 µg /ml.
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ab3686 at 5µg/ml staining of FEM 1B in
mouse liver tissue by IHC
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - FEM 1B antibody
(ab3686)
Immunofluorescence of F1A alpha in Mouse
Liver cells using ab3686 at 20 ug/ml.
Immunocytochemistry/ Immunofluorescence-AntiFEM 1B antibody(ab3686)
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