Product datasheet
Anti-APG5L/ATG5 antibody ab106551
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Overview
Product name
Anti-APG5L/ATG5 antibody
Description
Chicken polyclonal to APG5L/ATG5
Specificity
Three isoforms of APG5L/ATG5 are known to exist; ab106551 will only detect the longest
isoform.
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
A 16 amino acid synthetic peptide from near the N terminus of Human APG5L/ATG5
(EAW48415).
Positive control
Mouse spleen tissue lysate; Human spleen tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Applications
Our Abpromise guarantee covers the use of ab106551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
Use a concentration of 2.5 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa.
Target
Function
Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form
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cup-shaped isolation membrane and autophagosome. The conjugate detaches from the
membrane immediately before or after autophagosome formation is completed.
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its
expression is a relatively late event in the apoptotic process, occurring downstream of caspase
activity.
Tissue specificity
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the
protein is dramatically highly expressed in apoptotic cells.
Sequence similarities
Belongs to the ATG5 family.
Post-translational
modifications
Conjugated to ATG12; which is essential for autophagy, but is not required for association with
isolation membrane.
Cellular localization
Cytoplasm. Colocalizes with nonmuscle actin.
Anti-APG5L/ATG5 antibody images
Lane 1 : Anti-APG5L/ATG5 antibody
(ab106551) at 1 µg/ml
Lane 2 : Anti-APG5L/ATG5 antibody
(ab106551) at 2 µg/ml
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Western blot - APG5L/ATG5 antibody (ab106551)
Predicted band size : 32 kDa
ab106551, at 2.5µg/ml, staining
APG5L/ATG5 in Human spleen tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - APG5L/ATG5
antibody (ab106551)
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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