Product datasheet
Anti-Interferon alpha 6 antibody [EPR16355] ab192264
3 Images
Overview
Product name
Anti-Interferon alpha 6 antibody [EPR16355]
Description
Rabbit monoclonal [EPR16355] to Interferon alpha 6
Tested applications
WB, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human Interferon alpha 6 aa 50-150. The exact sequence is proprietary.
Database link: P05013
Positive control
U937, human fetal liver and human fetal lung lysates: Jurkat cells.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR16355
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab192264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
WB
Notes
1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular
weight: 22 kDa).
ICC/IF
1/50.
Flow Cyt
1/600.
Target
Relevance
Produced by macrophages, IFN alpha have mainly antiviral activities.
Cellular localization
Secreted
Anti-Interferon alpha 6 antibody [EPR16355] images
All lanes : Anti-Interferon alpha 6 antibody
[EPR16355] (ab192264) at 1/5000 dilution
Lane 1 : U937 cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase
Western blot - Anti-Interferon alpha 6 antibody
conjugated at 1/1000 dilution
[EPR16355] (ab192264)
Predicted band size : 22 kDa
Blocking/dilution buffer: 5% NFDM /TBST
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Immunofluorescent analysis of 4%
paraformaldehyde-fixed Jurkat cells labeling
Interferon alpha 6 with ab192264 at 1/50
dilution. The cells were permeabilised with
0.1% Triton X-100. Goat anti-rabbit IgG
(Alexa Fluor® 488) (ab150077) at 1/200
dilution was used as the secondary antibody
(green). The nuclear counter stain is DAPI
(blue).
The negative controls are as follows;
Immunocytochemistry/ Immunofluorescence -
1. Anti-Interferon alpha 6 at 1/50 dilution
Anti-Interferon alpha 6 antibody [EPR16355]
followed by Goat anti mouse IgG (Alexa
(ab192264)
Fluor®594) at 1/500 dilution.
2. Anti-Interferon alpha 6 at 1/50 dilution
followed by Goat anti mouse IgG (Alexa
Fluor®594) at 1/500 dilution.
Flow cytometry analysis of Jurkat cells
labelling Interferon alpha 6 (red) with purified
ab192264 at dilution of 1/600. The secondary
antibody used was Alexa Fluor® 488 goatanti-rabbit IgG (1/2000). Cells were fixed with
4% paraformaldehyde and permeabilised
with 90% methanol. Isotype control antibody
used was Rabbit Monoclonal IgG (black). The
blue line shows cells without incubation with
primary antibody and secondary antibody.
Flow Cytometry - Anti-Interferon alpha 6 antibody
[EPR16355] (ab192264)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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