Product datasheet
Anti-SP17 antibody [EP6496] ab172626
2 Images
Overview
Product name
Anti-SP17 antibody [EP6496]
Description
Rabbit monoclonal [EP6496] to SP17
Tested applications
ICC/IF, WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human SP17 aa 1-100. The exact sequence is proprietary.
Database link: Q15506
Positive control
Mouse testis, rat testis and Human testis lysates; Jurkat cells.
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP6496
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab172626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
ICC/IF
Abreviews
Notes
1/100 - 1/250.
1
Application
Abreviews
WB
Application notes
Notes
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
Is unsuitable for IHC-P or IP.
Target
Function
Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida
with high affinity. Might function in binding zona pellucida and carbohydrates.
Tissue specificity
Testis and sperm specific.
Sequence similarities
Contains 1 IQ domain.
Cellular localization
Membrane.
Anti-SP17 antibody [EP6496] images
Immunofluorescence analysis of Jurkat
cells staining SP17 with ab172626 at 1/100
dilution (green). DAPI nuclear staining (blue).
Immunocytochemistry/ Immunofluorescence Anti-SP17 [EP6496] antibody (ab172626)
All lanes : Anti-SP17 antibody [EP6496]
(ab172626) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 17 kDa
Western blot - Anti-SP17 [EP6496] antibody
(ab172626)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
2
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3