Product datasheet
Anti-Cullin 1 antibody [EPR3103Y] (Alexa Fluor® 488)
ab201514
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Overview
Product name
Anti-Cullin 1 antibody [EPR3103Y] (Alexa Fluor® 488)
Description
Rabbit monoclonal [EPR3103Y] to Cullin 1 (Alexa Fluor® 488)
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applications
ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
corresponding to Human Cullin 1 aa 750 to the C-terminus (C terminal).
Positive control
ICC/IF: HeLa cells.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
.
Alternative versions available:
Anti-Cullin 1 antibody [EPR3103Y] (ab75817)
Anti-Cullin 1 antibody (Alexa Fluor® 647) [EPR3103Y] (ab201016)
Anti-Cullin 1 antibody (HRP) [EPR3103Y] (ab201515)
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific
Company. The Alexa Fluor® dye included in this product is provided under an intellectual
property license from Life Technologies Corporation. As this product contains the Alexa Fluor®
dye, the purchase of this product conveys to the buyer the non-transferable right to use the
purchased product and components of the product only in research conducted by the buyer
(whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor®
dye the sale of this product is expressly conditioned on the buyer not using the product or its
components, or any materials made using the product or its components, in any activity to
generate revenue, which may include, but is not limited to use of the product or its components:
in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for
therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they
are sold for use in research. For information on purchasing a license to use products containing
Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation,
5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
EPR3103Y
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab201514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
ICC/IF
Abreviews
Notes
Use at an assay dependent concentration.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10
min) and 100% methanol (5 min).
Target
Function
Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitinprotein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle
progression, signal transduction and transcription. In the SCF complex, serves as a rigid
scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis
through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitinprotein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is
inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The
functional specificity of the SCF complex depends on the F-box protein as substrate recognition
component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in
Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC)
directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and
probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is
involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1,
RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs
ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably
PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of
MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs
ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53.
SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation
frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate
transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Tissue specificity
Expressed in lung fibroblasts.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the cullin family.
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Post-translational
modifications
Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction
with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1
leading to a S-phase-like environment that is required for efficient replication of the viral
genome.
Anti-Cullin 1 antibody [EPR3103Y] (Alexa Fluor® 488) images
ab201514 staining Cullin 1 in HeLa cells. The
cells were fixed with 4% formaldehyde (10
min), permeabilized with 0.1% Triton X-100
for 5 minutes and then blocked with 1%
BSA/10% normal goat serum/0.3M glycine in
0.1% PBS-Tween for 1h. The cells were then
incubated overnight at +4°C with ab201514 at
a 1/50 dilution (shown in green) and
ab195889, Mouse monoclonal to alpha
Tubulin (Alexa Fluor® 594), at a 1/250 dilution
(shown in red). Nuclear DNA was labelled
Immunocytochemistry/ Immunofluorescence -
with DAPI (shown in blue).
Anti-Cullin 1 antibody [EPR3103Y] (Alexa Fluor®
488) (ab201514)
Image was taken with a confocal microscope
(Leica-Microsystems, TCS SP8).
This product also gave a positive signal under
the same testing conditions in HeLa cells
fixed with 100% methanol (5 min).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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