Product datasheet
Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)]
ab124696
3 Images
Overview
Product name
Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)]
Description
Rabbit monoclonal [EPR2242(2)] to Cyclin E1 (phospho T77)
Specificity
ab124696 only detects Cyclin E1 phosphorylated at Threonine 77.
Tested applications
WB, IHC-P, ICC
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat
Immunogen
Synthetic peptide, corresponding to residues surrounding Threonine 77 of Human Cyclin E1.
Positive control
JAR cell lysate, treated with Lambda Phosphatase; Human colon tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 1.10 x 10 -12 M
Learn more about KD
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2242(2)
Isotype
IgG
1
Applications
Our Abpromise guarantee covers the use of ab124696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular
weight: 47 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with
IHC staining protocol.
ICC
Application notes
1/250 - 1/500.
Is unsuitable for IP.
Target
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Tissue specificity
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
Sequence similarities
Belongs to the cyclin family. Cyclin E subfamily.
Post-translational
modifications
Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the
ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Anti-Cyclin E1 (phospho T77) antibody [EPR2242(2)] images
All lanes : Anti-Cyclin E1 (phospho T77)
antibody [EPR2242(2)] (ab124696) at 1/1000
dilution
Lane 1 : JAR cell lysate, untreated
Lane 2 : JAR cell lysate, treated with Lambda
Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-Cyclin E1 (phospho T77)
Goat anti-Rabbit HRP at 1/2000 dilution
antibody [EPR2242(2)] (ab124696)
Predicted band size : 47 kDa
2
ab124696, at 1/100, staining Cyclin E1
(phospho T77) in paraffin-embedded Human
colon tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Cyclin E1
(phospho T77) antibody [EPR2242(2)] (ab124696)
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-Cyclin E1 (phospho T77) antibody
[EPR2242(2)](ab124696)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
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