Product datasheet
Anti-BMP7 antibody ab54904
1 Abreviews 3 Images
Overview
Product name
Anti-BMP7 antibody
Description
Mouse monoclonal to BMP7
Tested applications
WB, ICC/IF, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-432 of Human BMP7
Positive control
Purchase matching WB positive control:
Human BMP7 full length protein
This antibody gave a positive result in IHC in the following FFPE tissue: Human Kidney.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab54904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
ICC/IF
IHC-P
Application notes
WB: Use at a concentration of 1-5 µg/ml.
1
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the
phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone
homeostasis.
Tissue specificity
Expressed in the kidney and bladder. Lower levels seen in the brain.
Sequence similarities
Belongs to the TGF-beta family.
Developmental stage
Expressed in the developing eye, brain and ear during embryogenesis.
Post-translational
modifications
Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct
sequencing resulting in secretion of different mature forms (PubMed:17977014).
Cellular localization
Secreted.
Anti-BMP7 antibody images
2
IHC image of BMP7 staining in Human
Kidney formalin fixed paraffin embedded
tissue section, performed on a Leica Bond™
system using the standard protocol F. The
section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer
(pH6, epitope retrieval solution 1) for 20 mins.
The section was then incubated with
ab54904, 0.5 µg/ml, for 15 mins at room
temperature and detected using an HRP
conjugated compact polymer system. DAB
Immunohistochemistry (Formalin/PFA-fixed
was used as the chromogen. The section was
paraffin-embedded sections) - Anti-BMP7 antibody
then counterstained with haematoxylin and
(ab54904)
mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
Predicted band size : 49 kDa
BMP7 antibody (ab54904) at 1ug/lane + Raw
cell lysate at 25ug/lane.
Western blot - BMP7 antibody (ab54904)
3
ICC/IF image of ab54904 staining of BMP7 in
rat primary hippocampal neuron culture. The
sections were incubated in 0.3% triton X to
permeabilise the cells and in 10% serum to
block non-specific protein-protein
interactions. The sections were incubated
with ab54904 (1:1000) overnight at +4°C,
followed by a second incubation with Alexa
488 conjugated secondary antibody. BMP7
Immunocytochemistry/ Immunofluorescence -
(green staining) is observed in the pyramidal
Anti-BMP7 antibody (ab54904)
cells of the hippocampus.
This image was taken from an abreview submitted
by Ruma Raha-Chowdhury.
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