Product datasheet
Anti-ADFP antibody [2C5H8] ab181452
7 Images
Overview
Product name
Anti-ADFP antibody [2C5H8]
Description
Mouse monoclonal [2C5H8] to ADFP
Tested applications
IHC-P, WB, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Pig
Immunogen
Recombinant fragment corresponding to Human ADFP aa 268-437. Expressed in E. coli.
Sequence:
VEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGV
PQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESL
DDVMDYLVNNTPLNWLVGPFYPQLT ESQNAQDQGA
EMDKSSQETQ RSEHKTH
Database link: Q99541
Run BLAST with
Positive control
Run BLAST with
ADFP recombinant protein; ADFP transfected HEK293 cell lysate; HepG2 cells and cell lysate;
Human rectum cancer tissue; Human bladder cancer tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
0.5% stabilization buffer (amino acid =85% pH(1% water solution) =7.0~7.5, Water =2%
As(mg/kg) =0.5 Pb(mg/kg) =0.1)
Purity
Protein G purified
Clonality
Monoclonal
Clone number
2C5H8
Isotype
IgG1
Applications
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Our Abpromise guarantee covers the use of ab181452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000.
ICC/IF
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an
isotype control with this antibody.
Target
Function
May be involved in development and maintenance of adipose tissue.
Tissue specificity
Milk lipid globules.
Sequence similarities
Belongs to the perilipin family.
Post-translational
modifications
Acylated; primarily with C14, C16 and C18 fatty acids.
Cellular localization
Membrane.
Anti-ADFP antibody [2C5H8] images
Anti-ADFP antibody [2C5H8] (ab181452) at
1/500 dilution + HepG2 cell lysate
Western blot - Anti-ADFP [2C5H8] antibody
(ab181452)
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All lanes : Anti-ADFP antibody [2C5H8]
(ab181452) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : ADFP-transfected HEK293 cell
lysate
Western blot - Anti-ADFP [2C5H8] antibody
(ab181452)
Anti-ADFP antibody [2C5H8] (ab181452) at
1/500 dilution + ADFP recombinant protein
Western blot - Anti-ADFP [2C5H8] antibody
(ab181452)
Immunohistochemical analysis of paraffinembedded Human bladder cancer tissue
labeling ADFP with ab181452 at 1/200
dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ADFP [2C5H8]
antibody (ab181452)
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Immunohistochemical analysis of paraffinembedded Human rectum cancer tissue
labeling ADFP with ab181452 at 1/200
dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-ADFP [2C5H8]
antibody (ab181452)
Immunofluorescent analysis of HepG2 cells
labeling ADFP with ab181452 (green) at
1/200 dilution. Blue: DRAQ5 fluorescent DNA
dye.
Immunocytochemistry/ Immunofluorescence Anti-ADFP [2C5H8] antibody (ab181452)
Flow cytometric analysis of HepG2 cells
labeling ADFP with ab181452 (green) at
1/200 dilution, and negative control (red).
Flow Cytometry - Anti-ADFP [2C5H8] antibody
(ab181452)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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