Product datasheet
Anti-ABCG5 antibody ab87116
2 Images
Overview
Product name
Anti-ABCG5 antibody
Description
Rabbit polyclonal to ABCG5
Tested applications
ICC/IF, WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human
ABCG5.Read Abcam's proprietary immunogen policy(Peptide available as ab87830.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HepG2; SW480; Ramos.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab87116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa
(predicted molecular weight: 73 kDa).
Target
1
Function
Transporter that appears to play an indispensable role in the selective transport of the dietary
cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
Tissue specificity
Strongly expressed in the liver, lower levels in the small intestine and colon.
Involvement in disease
Defects in ABCG5 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as
phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized
by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols,
and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have
hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop
tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery
disease.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC
3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain.
Cellular localization
Membrane.
Anti-ABCG5 antibody images
All lanes : Anti-ABCG5 antibody (ab87116)
at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver
carcinoma cell line) Whole Cell Lysate
Lane 2 : SW480 (Human colon
adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : Ramos (Human Burkitt's lymphoma
cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Western blot - ABCG5 antibody (ab87116)
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) (ab65484) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 73 kDa
Observed band size : 74 kDa
Additional bands at : 12 kDa,68 kDa,70
kDa. We are unsure as to the identity of these
extra bands.
Exposure time : 1 minute
2
ICC/IF image of ab87116 stained MCF7
cells. The cells were 100% methanol fixed (5
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab87116, 5µg/ml) overnight
at +4°C. The secondary antibody (green) was
ab96899 Dylight® 488 goat anti-rabbit IgG
(H+L) used at a 1/250 dilution for 1h. Alexa
Immunocytochemistry/ Immunofluorescence -
Fluor® 594 WGA was used to label plasma
Anti-ABCG5 antibody (ab87116)
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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