Product datasheet
Anti-Angiogenin antibody [14017.7] ab10600
3 Abreviews 5 References 2 Images
Overview
Product name
Anti-Angiogenin antibody [14017.7]
Description
Mouse monoclonal [14017.7] to Angiogenin
Tested applications
ELISA, WB, ICC/IF, IHC-P, IHC-Fr
Species reactivity
Reacts with: Mouse, Human
Immunogen
Recombinant full length protein (Human).
Positive control
Purchase matching WB positive control:
Recombinant Human Angiogenin protein
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: 5% Trehalose, 0.% PBS
Purity
Protein G purified
Clonality
Monoclonal
Clone number
14017.7
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab10600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
Use a concentration of 1 - 2 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 14.4 kDa.
Non-reducing conditions and overnight incubation is recommended. Reducing
conditions can be used but often needs higher amounts of sample.
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Application
Abreviews
Notes
ICC/IF
1/500.
IHC-P
1/500.
IHC-Fr
Use at an assay dependent concentration.
Target
Function
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically
hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound,
angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization
of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in
vivo.
Tissue specificity
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
Involvement in disease
Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9)
[MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and
spinal cord. ALS is characterized by muscular weakness and atrophy.
Sequence similarities
Belongs to the pancreatic ribonuclease family.
Developmental stage
Low level expression in the developing fetus, increased in the neonate, and maximal in the adult.
Cellular localization
Secreted.
Anti-Angiogenin antibody [14017.7] images
ab10600 staining Angiogenin in murine
dermis (including follicle) by
Immunohistochemistry (Frozen sections).
Tissue was fixed with paraformaldehyde and
permeabilized using 0.1% Triton. Samples
were then blocked with 10% serum for 1 hour
at 19°C followed by incubation with the
primary antibody at a 1/500 dilution for 16
hours at 4°C. An Alexa Fluor®555 (red)
conjugated donkey anti-mouse polyclonal was
used as secondary antibody at a 1/200
Immunohistochemistry (Frozen sections) -
dilution. Counterstain DAPI (blue).
Angiogenin antibody [14017.7] (ab10600)
Image courtesy of an anonymous Abreview.
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Anti-Angiogenin antibody [14017.7]
(ab10600) at 2 µg/ml + Recombinant human
Angiogenin (E. coli derived) at 1 µg
Secondary
Anti-mouse IgG conjugated to HRP at 1/5000
dilution
developed using the ECL technique
Exposure time : 45 minutes
Western blot - Angiogenin antibody [14017.7]
Image courtesy of an anonymous Abreview.
(ab10600)
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