Product datasheet
Anti-CD36 antibody ab64014
3 Abreviews 2 Images
Overview
Product name
Anti-CD36 antibody
Description
Rabbit polyclonal to CD36
Tested applications
ICC/IF, WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human
CD36.Read Abcam's proprietary immunogen policy(Peptide available as ab99159.)
Positive control
This antibody gave a positive signal in Human Platelet Whole Cell Lysate, as well as Human
Spleen and Adipose tissue lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab64014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 85 kDa
(predicted molecular weight: 85 kDa).
Abcam recommends using BSA as the blocking agent.
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Target
Function
Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin,
anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly
mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain
fatty acids and may function in the transport and/or as a regulator of fatty acid transport.
Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic efects.
Involvement in disease
Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also
known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups.
The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting
complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in
platelets, but expression in monocytes/macrophages is near normal.
Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7
(CHDS7) [MIM:610938].
Sequence similarities
Belongs to the CD36 family.
Post-translational
modifications
N-glycosylated and O-glycosylated with a ratio of 2:1.
Cellular localization
Membrane.
Anti-CD36 antibody images
All lanes : Anti-CD36 antibody (ab64014) at
1 µg/ml
Lane 1 : Human Platelet (Human adult normal
cell line) Whole Cell Lysate
Lane 2 : Spleen (Human) Tissue Lysate adult normal tissue (ab29699)
Lane 3 : Adipose (Human) Total Protein
Lysate - adult normal tissue (ab28980)
Lysates/proteins at 10 µg per lane.
Western blot - CD36 antibody (ab64014)
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 85 kDa
Observed band size : 85 kDa
Additional bands at : 75 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 20 minutes
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All lanes : Anti-CD36 antibody (ab64014) at
2 µg/ml (Blocked in 3% milk)
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Adult Mouse Brown Adipose Tissue
Tissue Lysate
Lane 4 : Adult Rat Brown Adipose Tissue
Tissue Lysate
Lysates/proteins at 20 µg per lane.
Western blot - CD36 antibody (ab64014)
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
Predicted band size : 85 kDa
Observed band size : 85 kDa
Exposure time : 20 minutes
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