Product datasheet
Anti-BMP1 antibody ab118520
4 Abreviews 1 References 2 Images
Overview
Product name
Anti-BMP1 antibody
Description
Rabbit polyclonal to BMP1
Tested applications
IHC-P, ELISA
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Dog, Pig, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide:
RYTSTKFQDTLHSRK
conjugated to KLH, corresponding to C terminal amino acids 972-986 of Human BMP1.
Run BLAST with
Positive control
Run BLAST with
Human Skin and Colon tissues.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab118520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA
1/1 - 1/1000.
1
Target
Relevance
Bone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an
extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of
species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family
includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved
in extracellular matrix (ECM) formation, suggesting that a functional link may exist between
astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during
development. The name PCP reflects this enzyme’s involvement in the collagen deposition of
growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are
involved in the processing of fibrillar procollagen precursors to mature collagens, which is an
essential requirement for fibril formation. PCP cleaves the C-terminus from procollagen, to allow
the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen Nproteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder
dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and
connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at
the C-terminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have
extra EGF-like and CUB domains.
Cellular localization
Secreted
Anti-BMP1 antibody images
Immunohistochemical analysis of BMP1
expression in Formalin-Fixed, ParaffinEmbedded Human Skin using 10 µg/ml
ab118520 followed by biotinylated secondary
antibody, alkaline phosphatase-streptavidin
and chromogen.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-BMP1 antibody
(ab118520)
Immunohistochemical analysis of BMP1
expression in Formalin-Fixed, ParaffinEmbedded Human Colon using 10 µg/ml
ab118520 followed by biotinylated secondary
antibody, alkaline phosphatase-streptavidin
and chromogen.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-BMP1 antibody
(ab118520)
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