Product datasheet
Anti-UCP1 antibody ab23841
6 Abreviews 17 References 2 Images
Overview
Product name
Anti-UCP1 antibody
Description
Rabbit polyclonal to UCP1
Tested applications
WB, IHC-P, IHC-FoFr
Species reactivity
Reacts with: Mouse, Rat, Dog
Predicted to work with: Hamster, Human, Chimpanzee
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human UCP1.
Read Abcam's proprietary immunogen policy (Peptide available as ab24282.)
Positive control
WB: Mouse and rat brown adipose tissue lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab23841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa
(predicted molecular weight: 33 kDa).
IHC-P
Use at an assay dependent concentration.
IHC-FoFr
1/1000.
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Target
Function
UCP are mitochondrial transporter proteins that create proton leaks across the inner
mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a
result, energy is dissipated in the form of heat.
Tissue specificity
Brown adipose tissue.
Sequence similarities
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
Cellular localization
Mitochondrion inner membrane.
Form
UCP1 is preferentially expressed in brown adipose tissue
Anti-UCP1 antibody images
All lanes : Anti-UCP1 antibody (ab23841) at
1 µg/ml
Lane 1 : Adult Mouse Brown Adipose Tissue
Lysate
Lane 2 : Adult Rat Brown Adipose Tissue
Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Western blot - UCP1 antibody (ab23841)
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 33 kDa
Additional bands at : 100 kDa,50 kDa. We
are unsure as to the identity of these extra
bands.
Exposure time : 30 seconds
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ab23841 staining UCP1 in serial sections of
white fat from mice by immunohistochemistry.
Serial sections were de-paraffinized,
rehydrated through xylene, ethanol and water;
antigen retrieval was by heat mediation in a
Immunohistochemistry - Anti-UCP1 antibody
citrate buffer. Samples were incubated with
(ab23841)
primary antibody (1/200 in blocking buffer) for
Image from Liu W et al., PLoS Genet. 2006;8:1195863. Fig 5.; doi: 10.1371/journal.pgen.1003626
60 minutes. A HRP-conjugated anti-rabbit IgG
was used as the secondary antibody.
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