Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
Anti-CROT antibody
Rabbit polyclonal to CROT
Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Mouse, Human
Synthetic peptide corresponding to a region within N terminal amino acids 57-86 of Human
CROT, conjugated to KLH.
WB: K562 cell line and Mouse liver tissue lysates IHC-P: Human brain tissue Flow
Cytometry:K562 This antibody gave a positive result when used in the following formaldehyde fixed cell line: T24/83.
Properties
Form
Storage instructions
Storage buffer
Purity
Purification notes
Clonality
Isotype
Liquid
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Ammonium Sulphate Precipitation ab103448 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Polyclonal
IgG
Applications
Our Abpromise guarantee covers the use of ab103448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870 -Rabbit polyclonal IgG, is suitable for use as an isotype
WB control with this antibody.
1/50 - 1/100. Predicted molecular weight: 70 kDa.
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Application
IHC-P
ICC/IF
Target
Function
Pathway
Sequence similarities
Cellular localization
Abreviews Notes
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 5 µg/ml.
Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate.
Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.
Lipid metabolism; fatty acid beta-oxidation.
Belongs to the carnitine/choline acetyltransferase family.
Peroxisome.
Anti-CROT antibody images
Immunocytochemistry/ Immunofluorescence -
Anti-CROT antibody (ab103448)
ICC/IF image of ab103448 stained T24/83 cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10% normal goat serum/ 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103448 at 5 µg/mL overnight at +4°C. The secondary antibody
(green) was DyLight® 488 Goat anti-Rabbit
IgG (H+L) ( ab96899 ) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - CROT antibody (ab103448)
All lanes : Anti-CROT antibody (ab103448) at 1/50 dilution
Lane 1 : K562 cell lysate
Lane 2 : Mouse liver tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size : 70 kDa
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ab103448, at a 1/50 dilution, staining CROT in formalin fixed and paraffin embedded
Human brain tissue which was peroxidase conjugated to the secondary antibody, followed by DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CROT antibody
(ab103448)
Flow cytometric analysis of K562 cells with ab103448 at a 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goatanti-rabbit secondary antibodies were used for the analysis.
Flow Cytometry - CROT antibody (ab103448)
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