Product datasheet
Anti-COL4A3BP antibody ab72534
2 Images
Overview
Product name
Anti-COL4A3BP antibody
Description
Rabbit polyclonal to COL4A3BP
Tested applications
WB, IP
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey,
Gorilla, Opossum, Chinese Hamster, Orangutan, Elephant
Immunogen
Synthetic peptide from a region between residue 1 and 50 of human COL4A3BP
(NP_005704.1)
Positive control
Whole cell lysate from Hela and 293T cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab72534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
IP
Application notes
IP: Use at 1-4µg/mg of lysate.
WB: 1/2500 - 1/10000. Detects bands of approximately 70, 71, 110 and 230 kDa (predicted
molecular weight: 71 and 70 kDa for isoforms 1 and 2 respectively).
Not yet tested in other applications.
1
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
Tissue specificity
Widely expressed.
Sequence similarities
Contains 1 PH domain.
Contains 1 START domain.
Domain
The START domain recognizes ceramide and mediates the intermembrane transfer of
ceramide.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB.
Post-translational
modifications
Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at
Golgi membranes and reduces ceramide transfer activity.
Cellular localization
Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi
apparatus.
Anti-COL4A3BP antibody images
All lanes : Anti-COL4A3BP antibody
(ab72534) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at
50 µg
Lane 2 : Whole cell lysate from Hela cells at
15 µg
Lane 3 : Whole cell lysate from Hela cells at 5
µg
Lane 4 : Whole cell lysate from 293T cells at
50 µg
Western blot - COL4A3BP antibody (ab72534)
Predicted band size : 71 kDa
Observed band size : 70,71 kDa
Additional bands at : 110 kDa,230 kDa.
We are unsure as to the identity of these extra
bands.
2
Immunoprecipitation/ Western Blot of
COL4A3BP.
Lane 1: ab72534 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72534 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for
IP, 20% of IP loaded.
Chemiluminescence with an exposure time of
30 seconds.
Immunoprecipitation - COL4A3BP antibody
(ab72534)
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