Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
Anti-TBL1 antibody [4H2-D5-E9]
Mouse monoclonal [4H2-D5-E9] to TBL1
ICC/IF, WB, ELISA
Reacts with: Human
Recombinant full length protein corresponding to Human TBL1.
Database link: O60907
HeLa cell nuclear extract
Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Clone number
Isotype
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.4
Protein G purified
Monoclonal
4H2-D5-E9
IgG1
Applications
Our Abpromise guarantee covers the use of ab106150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
ICC/IF
WB
ELISA
1/50.
1/100 - 1/2000. Predicted molecular weight: 57 kDa.
1/5000 - 1/20000.
Target
1

Function
Tissue specificity
Sequence similarities
Domain
Cellular localization
F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
Ubiquitous.
Belongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats.
The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
Nucleus.
Anti-TBL1 antibody [4H2-D5-E9] images
Anti-TBL1 antibody [4H2-D5-E9] (ab106150) at 1/500 dilution + HeLa cell nuclear extract at
40 µg
Predicted band size : 57 kDa
Observed band size : 60 kDa
Western blot - TBL1Y antibody (ab106150)
Immunofluorescence staining of HeLa cells with anti-TBL1 antibody (ab106150) at a dilution of 1:100. Cells were fixed with 4% paraformaldehyde.
Immunocytochemistry/ Immunofluorescence -
Anti-TBL1 antibody [4H2-D5-E9] (ab106150)
2

All lanes : Anti-TBL1 antibody [4H2-D5-E9]
(ab106150) at 1/1000 dilution
Lane 1 : Y79 Cell Lysate
Lane 2 : Hela Cell Lysate
Predicted band size : 57 kDa
Western blot - Anti-TBL1Y antibody [4H2-D5-E9]
(ab106150)
Immunocytochemistry stain of Hela cells using ab106150(1:150).
Immunocytochemistry/ Immunofluorescence -
Anti-TBL1Y antibody [4H2-D5-E9] (ab106150)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3