Product datasheet
Anti-SULT2A1 antibody [EPR16095] - N-terminal
ab190690
3 Images
Overview
Product name
Anti-SULT2A1 antibody [EPR16095] - N-terminal
Description
Rabbit monoclonal [EPR16095] to SULT2A1 - N-terminal
Tested applications
WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human SULT2A1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q06520
Positive control
HepG2 and human adrenal gland tissue lysate. Human liver tissue. HepG2 cells.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR16095
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab190690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1
Application
Abreviews
WB
Notes
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular
weight: 34 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH
9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/200.
Target
Function
Catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands.
Tissue specificity
Liver, adrenal and at lower level in the kidney. Is present in human fetus in higher level in the
adrenal than the liver and the kidney.
Sequence similarities
Belongs to the sulfotransferase 1 family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Cytoplasm.
Anti-SULT2A1 antibody [EPR16095] - N-terminal images
All lanes : Anti-SULT2A1 antibody
[EPR16095] - N-terminal (ab190690) at
1/10000 dilution
Lane 1 : HepG2 cell line lysate
Lane 2 : Human adrenal gland tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase
Western blot - Anti-SULT2A1 [EPR16095]
conjugated antibody at 1/1000 dilution
antibody - N-terminal (ab190690)
Predicted band size : 34 kDa
Observed band size : 34 kDa
2
Immunofluorescent analysis of 4%
paraformaldehyde labeling SULT2A1 with
ab190690 at 1/100 followed by Goat anti
rabbit IgG(Alexa Fluor® 555) at 1/200 and
counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Anti-SULT2A1 [EPR16095] antibody - N-terminal
(ab190690)
Immunohistochemical analysis of paraffin
embedded human liver labeling SULT2A1
with ab190690 at 1/500 followed by HRP
conjugated secondary antibody and
counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-SULT2A1
[EPR16095] antibody - N-terminal (ab190690)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
3
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4