Product datasheet
Anti-Seladin 1 antibody ab40490
3 Abreviews 2 References 2 Images
Overview
Product name
Anti-Seladin 1 antibody
Description
Rabbit polyclonal to Seladin 1
Tested applications
WB
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Seladin
1.Read Abcam's proprietary immunogen policy(Peptide available as ab41486.)
Positive control
This antibody gave a positive signal in the following Human Tissue Lysates: Brain, Heart and
Skeletal Muscle.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab40490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa
(predicted molecular weight: 60 kDa).
Target
1
Function
Catalyzes the reduction of the delta-24 double bond of sterol intermediates. Protects cells from
oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress.
Also protects against amyloid-beta peptide-induced apoptosis.
Tissue specificity
Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen,
prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood
cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus,
hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression
in the inferior temporal lobe is substantially lower than in the frontal cortex.
Pathway
Steroid biosynthesis; cholesterol biosynthesis.
Involvement in disease
Desmosterolosis (DESMOS) [MIM:602398]: Rare autosomal recessive disorder characterized
by multiple congenital anomalies and elevated levels of the cholesterol precursor desmosterol in
plasma, tissue, and cultured cells. Note=The disease is caused by mutations affecting the gene
represented in this entry.
Sequence similarities
Belongs to the FAD-binding oxidoreductase/transferase type 4 family.
Contains 1 FAD-binding PCMH-type domain.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane.
Anti-Seladin 1 antibody images
All lanes : Anti-Seladin 1 antibody (ab40490)
at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult
normal tissue (ab29466)
Lane 2 : Heart (Human) Tissue Lysate - adult
normal tissue (ab29431)
Lane 3 : Skeletal Muscle (Human) Tissue
Lysate - adult normal tissue (ab29330)
Western blot - Seladin 1 antibody (ab40490)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 55 kDa
2
ICC/IF image of ab40490 stained HeLa cells.
The cells were 4% PFA fixed (10 min) and
then incubated in 1%BSA / 10% normal goat
serum / 0.3M glycine in 0.1% PBS-Tween for
1h to permeabilise the cells and block nonspecific protein-protein interactions. The cells
Immunocytochemistry/ Immunofluorescence Seladin 1 antibody (ab40490)
were then incubated with the antibody
(ab40490, 1µg/ml) overnight at +4°C. The
secondary antibody (green) was Alexa Fluor®
488 goat anti-rabbit IgG (H+L) used at a
1/1000 dilution for 1h. Alexa Fluor® 594 WGA
was used to label plasma membranes (red) at
a 1/200 dilution for 1h. DAPI was used to
stain the cell nuclei (blue) at a concentration of
1.43µM. This antibody also gave a positive
result in 4% PFA fixed (10 min) Hek293,
HepG2 and MCF7 cells at 1µg/ml.
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