Product datasheet
Anti-LXR alpha antibody [PPZ0412] - ChIP Grade
ab41902
11 Abreviews 12 References 4 Images
Overview
Product name
Anti-LXR alpha antibody [PPZ0412] - ChIP Grade
Description
Mouse monoclonal [PPZ0412] to LXR alpha - ChIP Grade
Specificity
This antibody does not recognize human LXR beta.
Tested applications
ChIP, ICC/IF, WB, ELISA, IP, Gel supershift assays, IHC-P, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
E.coli-expressed recombinant fragment, corresponding to amino acids 164-447 of Human LXR
alpha.
Positive control
Rat hepatocyte and kupffer cell. Rat spleen macrophage.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.1% Sodium azide
Physiological saline.
Purification notes
Purified form ascites via ammonium sulfate fractionation.
Clonality
Monoclonal
Clone number
PPZ0412
Isotype
IgG2a
Applications
Our Abpromise guarantee covers the use of ab41902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ChIP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
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Application
Abreviews
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ELISA
Use a concentration of 0.2 µg/ml.
IP
Use at an assay dependent concentration.
Gel supershift assays
Use at an assay dependent concentration.
IHC-P
Use a concentration of 20 - 40 µg/ml.
Flow Cyt
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as
an isotype control with this antibody.
Target
Function
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to
an active ligand-binding subunit in mediating retinoid responses through target genes defined by
LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar
hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of
cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination
of LDLR, VLDLR and LRP8.
Tissue specificity
Visceral organs specific expression. Strong expression was found in liver, kidney and intestine
followed by spleen and to a lesser extent the adrenals.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Cellular localization
Nucleus.
Anti-LXR alpha antibody [PPZ0412] - ChIP Grade images
ab41902 staining LXR alpha in Rat
Hepatocyte and Kupffer cell paraffin section
(20-40ug/mL) by Immunohistochemistry,
formalin-fixed paraffin embedded sections.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - LXR alpha antibody
[PPZ0412] (ab41902)
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ab41902 staining LXR alpha in rat spleen
macrophage cell paraffin section (2040ug/mL) by Immunohistochemistry, formalinfixed paraffin embedded sections.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - LXR alpha antibody
[PPZ0412] (ab41902)
All lanes : Anti-LXR alpha antibody
[PPZ0412] - ChIP Grade (ab41902) at
1/1000 dilution (in 2.5% BSA + TBS for 16
hours at 4°C)
Lane 1 : RAW 264.7 murine macrophage cell
line lysate without endogenous LXRalpha
Western blot - LXR alpha antibody [PPZ0412] -
Lane 2 : RAW 264.7 murine macrophage cell
ChIP Grade (ab41902)
line lysate overexpressing human FLAGLXRalpha
Lysates/proteins at 35 µg per lane.
Secondary
An HRP-conjugated Goat anti-mouse IgG
polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Exposure time : 1 minute
This image is courtesy of an anonymous
Abreview
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Overlay histogram showing HepG2 cells
stained with ab41902 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry-LXR alpha antibody [PPZ0412] ChIP Grade(ab41902)
(ab41902, 1µg/1x106 cells) for 30 min at
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG2a [ICIGG2A] (ab91361,
2µg/1x106 cells) used under the same
conditions. Acquisition of >5,000 events was
performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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