Product datasheet Recombinant human EZH1 + EED + SUZ12 protein ab196395 2 Images Overview Product name Recombinant human EZH1 + EED + SUZ12 protein Protein length Full length protein Description Nature Recombinant Source Baculovirus Amino Acid Sequence 1 Accession Q92800 Species Human Sequence EIPNPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQE KTQILNEEWKKLRVQPVQSMKPVSGHPFLKKCTIESIFPGFASQHMLMRS LNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELI NNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEEEEGHNDTSDGK QDDSKEDLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPD DMKERYRELTEMSDPNALPPQCTPNIDGPNAKSVQREQSLHSFHTLFCRR CFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEYAMLH NPRSKCSGRRRRRHHIVSASCSNASASAVAETKEGDSDRDTGNDWASSSS EANSRCQTPTKQKASPAPPQLCVVEAPSEPVEWTGAEESLFRVFHGTYFN NFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELMNPSQKKKRKHRLWA AHCRKIQLKKDNSSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCN PDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVS CKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDE ADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVV MVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVL Molecular weight 86 kDa including tags Amino acids 2 to 747 Tags His tag N-Terminus Amino Acid Sequence 2 Accession O75530 Species Human Sequence SEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTE RPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQF 1 NWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCA WTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPR DPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKI MSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFST RDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVT ILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKC TTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR Molecular weight 51 kDa including tags Amino acids 2 to 441 Tags DDDDK tag N-Terminus Amino Acid Sequence 3 Accession Q15022 Species Human Sequence APQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGS YSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLR TRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESH SLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSC PIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLL FRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTV FDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQ GPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIA VKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCP WCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAG NPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEV EQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIE EFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKN LCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEI TEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL Molecular weight 87 kDa including tags Amino acids 2 to 739 Tags His tag N-Terminus Specifications Our Abpromise guarantee covers the use of ab196395 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Biological activity Specific activity: ≥0.0005 pmol/min/µg. Applications Functional Studies SDS-PAGE Purity >= 70 % by SDS-PAGE. Form Liquid Additional notes Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Preparation and Storage 2 Stability and Storage Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. pH: 8.00 Constituents: 0.63% Tris HCl, 0.06% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol 80 ng/µL FLAG peptide This product is an active protein and may elicit a biological response in vivo, handle with caution. General Info Cellular localization Nuclear Recombinant human EZH1 + EED + SUZ12 protein images Example of specific activity of ab196395. Functional Studies - Active human EZH1 + EED + SUZ12 full length protein (ab196395) 4-20% SDS PAGE with Coomassie staining: 3.5 µg ab196395. SDS-PAGE - Active human EZH1 + EED + SUZ12 full length protein (ab196395) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. 3 Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 4