Gen Bio II Laboratory 14: Bacteria and Protists p. 5 ***Note: Students will NOT be doing the Gram staining method. Prepared Gram stained slides are available for this lab. Gram staining procedures are reserved for advanced biology courses due to safety and liability. Per Lab: 3 types bacteria slides 33 Gloeocapsa slides 77 Oscillatoria slides 79 Anabaena slides 78 Algae slides 0 Volvox slides 13 Spirogyra slides 14 Preserved red, brown, and green algae Diatom slides 16 Dinoflagellate slides 15 Slime Mold 132 Immersion oil Per Table: Start bacteria swab plates (2 nights before lab) and physarum (a few weeks) in advance. Live Physarum (1 plate/ table. Make 6 plates/semester and re-use for all labs.) Cultured agar plate (Any bacteria. Make 6 plates/semester and re-use for all labs.) Sterile nutrient agar plate to incubate after student inoculation Sterile swabs Clean slides Coverslips Stereoscope China marker or sharpie 1% Bleach beaker Gen Bio II Laboratory 15: Fungi p. 25 Per Lab: Rhizopus slides 23 Yeast slides 101,102 Peziza slides 22 Fungi sample specimens Live Aspergillus (1 plate/semester to re-use for all sections) Penicillium slides 126 AP Aspergillus slides 82 AP Edible mushrooms Coprinus slides 24, 83 Lichen samples Lichen slides 25 Mycorriza slides 0 Cultured agar plates from last week 10% bleach in 800-1000mL beaker Per Table: Moldy bread (Start a month before.) Methylene blue in dropper bottles Clean slides Clear tape Gen Bio II Laboratory 16: Nonvascular Plants and Seedless Vascular Plants p. 37 Per Lab: Live Chara (1/semester for demo only) Preserved mosses Moss slides 121 Live Marchantia (1/semester for demo only) Live Selaginella Live Lycopodium Preserved Lycopodium Preserved Selaginella Lycopodium slides 122 Selaginella slides 122 Preserved Psilotum Live Equisetum Fern frond cross section slides 123 Fern slides 123 Gen Bio II Laboratory 17: Seed Plants p. 53 Per Lab: Supplies for 17.1-17.2: Zamia slides 109 Cycas slides 111 Pine needles Pine cones Pine cone slides 66, 75 Supplies for 17.3-17.4: Fresh flowers ( 1/table, but at least 1 monocot and one dicot/lab) Flower model Pollen grain slides 68 Mature embryo slides 69 10% bleach in 800-1000mL beaker Per Table: Supplies for 17.2: Pine nuts All sections: Dissection tools Supplies for 17.3: Clean slides Coverslips Note: Monocots include astrolomeria, lilies, daffodils, iris, orchids, bluebells, tulips. Dicots include geranium, snapdragon, magnolias, water lily, star anise, hornwort. Gen Bio II Laboratory 22: Introduction to Invertebrates p. 69 Per Lab: Supplies for 22.1-22.3: Preserved sponges (Get these from Bruce. He keeps them in his office.) Grantia slides Sponge slides 83, 84 Hydra slides 87 Live Hydra (Don’t order Hydra from Ward’s) Jellyfish, coral,and sponge specimens and plastimounts Supplies for 22.4-22.6: This is a different lab! Live Planaria Planaria slides Preserved roundworms Earthworms (Dr. Coots only) Live rotifers 10% bleach in 800-1000mL beaker Per Table: Supplies for 22.4-22.6: Same as above lab! Dissection tools Penlights All sections: Petri dishes Supplies for 22.4-22.6: Liver for planaria food source. Concave depression slides Coverslips Ascaris for dissection (male and female) Ascaris slides 93 Gen Bio II Chapter 12: Annelida p. 97 Per Lab: Preserved leeches Earthworm slides 92 Earthworm anatomy model 10% bleach in 800-1000mL beaker Per Table: Earthworm for dissection Clean slides Coverslips Earthworm anatomy charts Gen Bio II Chapter 11: Mollusca p. 113 Per Lab: Preserved mollusks Mollusk shells Per Table: Squid for dissection Dissection tools Squid anatomy chart Petri dishes for ink Gen Bio II Chapter 14: Echinodermata p. 131 Per Lab: Preserved specimens o Sea stars o Sea urchins o Sand dollars o Sea cucumber Sea star ray slides 95 Sea star-bipnnaria larva slides 95 Sea star anatomy model Per Table: Sea star for dissection Dissection tools Sea star anatomy charts (if we have them) Clean slides Coverslips Gen Bio II Chapter 16: Shark Anatomy p. 141 Per Lab: Placoid scale slides 119 Fish gill slides 120 Fish skin slides 118 Per Table: Shark for dissection Dissection tools Shark anatomy charts -none Gen Bio II Chapter 20: Rat Anatomy p. 229 Per Lab: Rat anatomy model Testis slides 45AP Kidney slides 21 AP Heart slides 42 AP (Cardiac Muscle) Mounted rat skeleton Per Table: Rat for dissection Dissection tools Rat anatomy charts