Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Anti-NCF1 antibody
Goat polyclonal to NCF1
WB, ELISA, IHC-P
Reacts with: Human
Predicted to work with: Cow, Pig
Synthetic peptide:
CSESTKRKLASAV conjugated to KLH, corresponding to C-terminal amino acids 378-390 of Human NCF1
(NP_000256.3).
Run BLAST with Run BLAST with
U937 lysate, Human Colon tissue.
Positive control
Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Isotype
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Protein G purified
Polyclonal
IgG
Applications
Our Abpromise guarantee covers the use of ab115564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
ELISA
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 45 kDa.
Use at an assay dependent dilution.
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Application
IHC-P
Abreviews Notes
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Involvement in disease
Sequence similarities
Cellular localization
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent
NADPH oxidase (necessary for superoxide production).
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains.
Cytoplasm.
Anti-NCF1 antibody images
Anti-NCF1 antibody (ab115564) at 0.2 µg/ml
+ U937 lysate (in RIPA buffer) at 30 µg developed using the ECL technique
Predicted band size : 45 kDa
Western blot - Anti-NCF1 antibody (ab115564)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1 antibody
(ab115564) ab115564, at 5µg/ml, staining NCF1 in
Formalin-fixed, Paraffin-embedded Human
Colon (neutrophils) tissue by
Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
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Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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