Product datasheet
Anti-Non Neuronal Enolase antibody ab54979
2 Abreviews 1 References 4 Images
Overview
Product name
Anti-Non Neuronal Enolase antibody
Description
Mouse monoclonal to Non Neuronal Enolase
Tested applications
Flow Cyt, WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-435 of Human Non Neuronal
Enolase
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab54979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Flow Cyt
Abreviews
Notes
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
an isotype control with this antibody.
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
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Target
Function
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes
such as growth control, hypoxia tolerance and allergic responses. May also function in the
intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and
activator of plasminogen on the cell surface of several cell-types such as leukocytes and
neurons. Stimulates immunoglobulin production.
MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor
suppressor.
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta
heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma
heterodimer and the gamma/gamma homodimer in neurons.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similarities
Belongs to the enolase family.
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta
heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Post-translational
modifications
ISGylated.
Cellular localization
Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can
translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric
(alpha/gamma) form. ENO1 is localized to the M line.
Anti-Non Neuronal Enolase antibody images
Non Neuronal Enolase antibody (ab54979)
used in immunohistochemistry at 5ug/ml on
formalin fixed and paraffin embedded human
lymphoma tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Non Neuronal
Enolase antibody (ab54979)
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Predicted band size : 47 kDa
Western blot - Non Neuronal Enolase antibody
(ab54979)
ICC/IF image of ab54979 stained HeLa cells.
The cells were 4% formaldehyde fixed (10
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab54979, 1µg/ml) overnight
at +4°C. The secondary antibody (green) was
Alexa Fluor® 488 goat anti-mouse IgG (H+L)
used at a 1/1000 dilution for 1h. Alexa Fluor®
Immunocytochemistry/ Immunofluorescence-Non
594 WGA was used to label plasma
Neuronal Enolase antibody(ab54979)
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
Overlay histogram showing HeLa cells
stained with ab54979 (red line). The cells
were fixed with 4% paraformaldehyde (10
min) and then permeabilized with 0.1% PBSTween for 20 min. The cells were then
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
protein-protein interactions followed by the
Flow Cytometry-Anti-Non Neuronal Enolase
antibody(ab54979)
antibody (ab54979, 1µg/1x106 cells) for 30
min at 22ºC. The secondary antibody used
was DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106
cells) used under the same conditions.
Acquisition of >5,000 events was performed.
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