Product datasheet
Anti-MDH1 antibody [2B11-B7] ab55528
3 Abreviews 3 Images
Overview
Product name
Anti-MDH1 antibody [2B11-B7]
Description
Mouse monoclonal [2B11-B7] to MDH1
Tested applications
WB, IP, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-335 of Human Malate
Dehydrogenase 1 (MDH1).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Purity
Protein G purified
Clonality
Monoclonal
Clone number
2B11-B7
Isotype
IgG2b
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab55528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 36 kDa.
IP
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as
an isotype control with this antibody.
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Target
Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 2 family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Anti-MDH1 antibody [2B11-B7] images
Predicted band size : 36 kDa
Western blot - Anti-MDH1 antibody [2B11-B7]
(ab55528)
Overlay histogram showing HL60 cells
stained with ab55528 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions. The cells were then incubated
Flow Cytometry - Anti-MDH1 antibody [2B11-B7]
with the antibody (ab55528, 1µg/1x106 cells)
(ab55528)
for 30 min at 22ºC. The secondary antibody
used was DyLight® 488 goat anti-mouse IgG
(H+L) (ab96879) at 1/500 dilution for 30 min
at 22ºC. Isotype control antibody (black line)
was mouse IgG2b [PLPV219] (ab91366,
2µg/1x106 cells) used under the same
conditions. Acquisition of >5,000 events was
performed. This antibody gave a positive
signal in HL60 cells fixed with 4%
paraformaldehyde (10 min)/permeabilized
with 0.1% PBS-Tween for 20 min used under
the same conditions.
2
Detection of MDH1 by Immunoprecipitation.
ab55528 used at 5µl/mg for 500µg of Mouse
whole cell lysate from Hippocampal neurol
cells for IP using Dynabeads for 16 hours at 4
degrees (in PBST). Immunoprecipitated
MAD2 was blotted with another antibody
HRP-congugated Goat anti-mouse Polyclonal
at 1/5000. Detection: Chemiluminescence
with an exposure time of 1 minute. Band size
Immunoprecipitation - Anti-MDH1 antibody [2B11-
36kDa.
B7] (ab55528)
This image is courtesy of an anonymous Abreview
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