Product datasheet
Anti-Lactate Dehydrogenase antibody [98A-1F9BB1]
ab135396
1 References 5 Images
Overview
Product name
Anti-Lactate Dehydrogenase antibody [98A-1F9BB1]
Description
Mouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase
Tested applications
WB, Flow Cyt, ICC, IP, In-Cell ELISA
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Human
Does not react with: Cow
Immunogen
Rabbit muscle Lactate Dehydrogenase.
Positive control
Human, mouse and rat liver and heart homogenates; HDFn, HeLa, HepG2, H9C2, H4IIE and
MEF whole cell lysates; Lactate Dehydrogenase purified from rabbit skeletal muscle; HeLa cells.
General notes
Product was previously marketed under the MitoSciences sub-brand.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.357% HEPES, 0.877% Sodium chloride
Purity
Ammonium Sulphate Precipitation
Clonality
Monoclonal
Clone number
98A-1F9BB1
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab135396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
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Application
Abreviews
Flow Cyt
Notes
Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable
for use as an isotype control with this antibody.
ICC
Use a concentration of 1 µg/ml.
IP
Use a concentration of 1 µg/ml.
In-Cell ELISA
Use a concentration of 1 µg/ml.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A
metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] images
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All lanes : Anti-Lactate Dehydrogenase
antibody [98A-1F9BB1] (ab135396) at 1
µg/ml
Lane 1 : Ladder
Lane 2 : Human liver homogenate at 15 µg
Lane 3 : Human heart homogenate at 15 µg
Western blot - Anti-Lactate Dehydrogenase
Lane 4 : Rat liver homogenate at 15 µg
antibody [98A-1F9BB1] (ab135396)
Lane 5 : Rat heart homogenate at 15 µg
Lane 6 : Mouse liver homogenate at 15 µg
Lane 7 : Mouse heart homogenate at 15 µg
Lane 8 : Ladder
Lane 9 : Human fibroblast whole cell lysate at
15 µg
Lane 10 : HeLa whole Cell Lysate at 15 µg
Lane 11 : HepG2 whole cell lysate at 15 µg
Lane 12 : H9C2 (Rat cardiomyoblast cell
line) whole Cell Lysate at 15 µg
Lane 13 : H4IIE (Rat hepatoma cell line)
whole Cell Lysate at 15 µg
Lane 14 : MEF (Mouse embryonic fibroblast
cell line) whole Cell Lysate at 15 µg
Lane 15 : LDH (purified from rabbit skeletal
muscle) at 0.015 µg
Secondary
Goat polyclonal to Mouse IgG - AP at 1/3000
dilution
Predicted band size : 37 kDa
Flow cytometric analysis of HeLa cells (fixed
and permeabilized with methanol) labelling
Lactate Dehydrogenase with ab135396 at 1
µg/ml (blue) or an equal amount of an isotype
control antibody (red). 1% BSA was used as
the blocking reagent for all the blocking steps.
Flow Cytometry - Anti-Lactate Dehydrogenase
antibody [98A-1F9BB1] (ab135396)
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Immunocytochemistry analysis of
Paraformaldehyde fixed Hela cells labelling
Lactate Dehydrogenase with ab135396 at 1
µg/ml. Detection utilised Alexa 594 goat antimouse IgG (H+L) used at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence Anti-Lactate Dehydrogenase antibody [MS#98A1F9BB1] (ab135396)
Immunoprecipitation of Lactate
Dehydrogenase from 1mg of Hela Cell Lysate
with ab135396 at 1 µg/ml. The gel was
stained with Coomassie brilliant blue G.
Immunoprecipitation - Anti-Lactate
Dehydrogenase antibody [98A-1F9BB1]
(ab135396)
In-cell ELISA analysis of
paraformaldehyde/PBS fixed HeLa cells
seeded at 20k cells/well. ab135396 was
serially diluted in 1%BSA/PBS and detected
with goat anti-mouse dye-conjugated antibody
at 1/1000 in 1%BSA/PBS.
In-Cell ELISA - Anti-Lactate Dehydrogenase
antibody [MS#98A-1F9BB1] (ab135396)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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