Product datasheet Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] ab135396 1 References 5 Images Overview Product name Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] Description Mouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase Tested applications WB, Flow Cyt, ICC, IP, In-Cell ELISA Species reactivity Reacts with: Mouse, Rat, Rabbit, Human Does not react with: Cow Immunogen Rabbit muscle Lactate Dehydrogenase. Positive control Human, mouse and rat liver and heart homogenates; HDFn, HeLa, HepG2, H9C2, H4IIE and MEF whole cell lysates; Lactate Dehydrogenase purified from rabbit skeletal muscle; HeLa cells. General notes Product was previously marketed under the MitoSciences sub-brand. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Storage buffer pH: 7.30 Preservative: 0.02% Sodium azide Constituents: 0.357% HEPES, 0.877% Sodium chloride Purity Ammonium Sulphate Precipitation Clonality Monoclonal Clone number 98A-1F9BB1 Isotype IgG1 Applications Our Abpromise guarantee covers the use of ab135396 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. 1 Application Abreviews Flow Cyt Notes Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. ICC Use a concentration of 1 µg/ml. IP Use a concentration of 1 µg/ml. In-Cell ELISA Use a concentration of 1 µg/ml. Target Pathway Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. Involvement in disease Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. Sequence similarities Belongs to the LDH/MDH superfamily. LDH family. Post-translational modifications ISGylated. Cellular localization Cytoplasm. Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] images 2 All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/ml Lane 1 : Ladder Lane 2 : Human liver homogenate at 15 µg Lane 3 : Human heart homogenate at 15 µg Western blot - Anti-Lactate Dehydrogenase Lane 4 : Rat liver homogenate at 15 µg antibody [98A-1F9BB1] (ab135396) Lane 5 : Rat heart homogenate at 15 µg Lane 6 : Mouse liver homogenate at 15 µg Lane 7 : Mouse heart homogenate at 15 µg Lane 8 : Ladder Lane 9 : Human fibroblast whole cell lysate at 15 µg Lane 10 : HeLa whole Cell Lysate at 15 µg Lane 11 : HepG2 whole cell lysate at 15 µg Lane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg Lane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg Lane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg Lane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µg Secondary Goat polyclonal to Mouse IgG - AP at 1/3000 dilution Predicted band size : 37 kDa Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps. Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) 3 Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat antimouse IgG (H+L) used at 1/1000 dilution. Immunocytochemistry/ Immunofluorescence Anti-Lactate Dehydrogenase antibody [MS#98A1F9BB1] (ab135396) Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G. Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS. In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [MS#98A-1F9BB1] (ab135396) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support 4 Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 5