Product datasheet
Anti-CACNA1A antibody [EPR4458(N)] ab181371
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Overview
Product name
Anti-CACNA1A antibody [EPR4458(N)]
Description
Rabbit monoclonal [EPR4458(N)] to CACNA1A
Tested applications
WB, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human CACNA1A aa 2050-2150 (Cysteine residue). The exact sequence is proprietary.
Database link: O00555
Positive control
Human fetal brain, Human cerebellum and C6 lysates; Permeabilized C6 cells.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR4458(N)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab181371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 170-282 kDa (predicted
molecular weight: 282 kDa).Can be blocked with CACNA1A peptide
(ab205923).
Flow Cyt
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype
control with this antibody.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
Relevance
Cav2.1 is a voltage-sensitive calcium channels (VSCC) which belongs to the calcium channel
alpha-1 subunit family. Cav2.1 mediates the entry of calcium ions into excitable cells and is also
involved in a variety of calcium-dependent processes, including muscle contraction, hormone or
neurotransmitter release, gene expression, cell motility, cell division and cell death. Cav2.1
(isoform alpha-1A) gives rise to P and/or Q-type calcium currents. Voltage-dependent calcium
channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a
1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1
subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel
activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the
channel activity.
Cellular localization
Cell Membrane
Anti-CACNA1A antibody [EPR4458(N)] images
All lanes : Anti-CACNA1A antibody
[EPR4458(N)] (ab181371) at 1/1000 dilution
Lane 1 : Human fetal brain lysates
Lane 2 : Human cerebellum lysates
Lane 3 : C6 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size : 282 kDa
Western blot - Anti-CACNA1A [EPR4458(N)]
antibody (ab181371)
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Flow cytometric analysis of permeabilized C6
cells labeling CACNA1A with ab181371 at
1/10 dilution (red) or a rabbit IgG (negative)
(green).
Flow Cytometry - Anti-CACNA1A [EPR4458(N)]
antibody (ab181371)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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