Product datasheet
Anti-Superoxide Dismutase 3 antibody [1H12] ab28442
1 References 2 Images
Overview
Product name
Anti-Superoxide Dismutase 3 antibody [1H12]
Description
Mouse monoclonal [1H12] to Superoxide Dismutase 3
Tested applications
WB, IP, IHC-P, ELISA
Species reactivity
Reacts with: Human
Immunogen
Recombinant full length protein (Human) purified from E.coli.
Positive control
Bosc23 cell lysate transfected with Myc-His-SOD3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Purity
Protein A purified
Clonality
Monoclonal
Clone number
1H12
Isotype
IgG2b
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab28442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/2000. Predicted molecular weight: 26 kDa.
IP
Use at 1 µg/mg of lysate.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
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Target
Function
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting
superoxide radicals into hydrogen peroxide and oxygen.
Tissue specificity
Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in
extracellular fluids such as plasma, lymph and synovial fluid.
Sequence similarities
Belongs to the Cu-Zn superoxide dismutase family.
Cellular localization
Secreted > extracellular space. 99% of EC-SOD is anchored to heparan sulfate proteoglycans
in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma
and the endothelium.
Anti-Superoxide Dismutase 3 antibody [1H12] images
All lanes : Anti-Superoxide Dismutase 3
antibody [1H12] (ab28442)
Lane 1 : Bosc23 cell lysate
Lane 2 : Bosc23 cell lysate transfected with
Myc-His-SOD3
Lane 3 : Bosc23 cell lysate transfected with
Myc-His-Gpx3
Western blot - Superoxide Dismutase 3 antibody
Predicted band size : 26 kDa
[1H12] (ab28442)
Observed band size : 35 kDa
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IHC image of ab28442 staining in human
heart formalin fixed paraffin embedded tissue
section, performed on a Leica BondTM
system using the standard protocol F. The
section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer
(pH6, epitope retrieval solution 1) for 20 mins.
The section was then incubated with
ab28442, 1µg/ml, for 15 mins at room
Immunohistochemistry (Formalin/PFA-fixed
temperature and detected using an HRP
paraffin-embedded sections)-Superoxide
conjugated compact polymer system. DAB
Dismutase 3 antibody [1H12](ab28442)
was used as the chromogen. The section was
then counterstained with haematoxylin and
mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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