Product datasheet
Anti-Cytochrome C Oxidase subunit VIb antibody
[3F9D3D11AF6] (HRP) ab198317
2 Images
Overview
Product name
Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (HRP)
Description
Mouse monoclonal [3F9D3D11AF6] to Cytochrome C Oxidase subunit VIb (HRP)
Conjugation
HRP
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
Immunogen
Bovine Complex IV (native holoenzyme protein, purified from heart)
Positive control
WB: Human Heart Mitochondrial Lysate IHC-P: normal human heart muscle tissue sections.
General notes
Alternative versions:
Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (ab110266)
Anti-Cytochrome C Oxidase subunit VIb antibody (Alexa Fluor® 488) [3F9D3D11AF6]
(ab198315)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Purity
Immunogen affinity purified
Purification notes
Near homogeneity as judged by SDS-PAGE. This antibody was produced in vitro using
hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
3F9D3D11AF6
Isotype
IgG1
Light chain type
kappa
1
Applications
Our Abpromise guarantee covers the use of ab198317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 10 kDa (predicted molecular weight: 10
kDa).
Target
Function
Connects the two COX monomers into the physiological dimeric form.
Involvement in disease
Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D)
[MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial
respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to
severe multisystem disease affecting several tissues and organs. Features include hypertrophic
cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise
intolerance, developmental delay, delayed motor development and mental retardation. A subset
of patients manifest Leigh syndrome.
Sequence similarities
Belongs to the cytochrome c oxidase subunit 6B family.
Cellular localization
Mitochondrion intermembrane space.
Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6] (HRP) images
2
Anti-Cytochrome C Oxidase subunit VIb
antibody [3F9D3D11AF6] (HRP) (ab198317)
at 1/5000 dilution + Human Heart
Mitochondrial lysate (ab110337) at 5 µg
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 10 kDa
Observed band size : 10 kDa
Western blot - Anti-Cytochrome C Oxidase
subunit VIb antibody [3F9D3D11AF6] (HRP)
Exposure time : 10 seconds
(ab198317)
This blot was produced using a 4-12% Bistris gel under the MES buffer system. The gel
was run at 200V for 35 minutes before being
transferred onto a Nitrocellulose membrane at
30V for 70 minutes. The membrane was then
blocked for an hour using 2% Bovine Serum
Albumin before being incubated with
ab198317 overnight at 4°C. Antibody binding
was visualised using ECL development
solution ab133406.
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IHC image of Cytochrome c oxidase subunit
staining in a section of formalin-fixed paraffinembedded normal human heart muscle
tissue*, performed on a Leica BOND. The
section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer
(pH6, epitope retrieval solution 1) for 20mins.
The section was then incubated with
ab198317 at 1/100 dilution, for 15 mins at
room temperature. DAB was used as the
chromogen. The section was then
Immunohistochemistry (Formalin/PFA-fixed
counterstained with haematoxylin and
paraffin-embedded sections) - Anti-Cytochrome C
mounted with DPX. The inset negative control
Oxidase subunit VIb antibody [3F9D3D11AF6]
image is taken from an identical assay
(HRP) (ab198317)
without primary antibody.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
*Tissue obtained from the Human Research
Tissue Bank, supported by the NIHR
Cambridge Biomedical Research Centre
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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